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{{STRUCTURE_4ce1|  PDB=4ce1  |  SCENE=  }}
==Hsp90 N-terminal domain bound to macrolactam analogues of radicicol.==
===Hsp90 N-terminal domain bound to macrolactam analogues of radicicol.===
<StructureSection load='4ce1' size='340' side='right' caption='[[4ce1]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
{{ABSTRACT_PUBMED_24435512}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4ce1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CE1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CE1 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=7FK:15-CHLORO-16,18-DIHYDROXY-2-METHYL-3,4,7,8,9,10,11,12-OCTAHYDROBENZ[C][1]AZACYCLOHEXADECINE-1,13(2H,14H)-DIONE'>7FK</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ce2|4ce2]], [[4ce3|4ce3]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ce1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ce1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ce1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ce1 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A series of macrolactam analogues of the naturally occurring resorcylic acid lactone radicicol have been synthesised from methyl orsellinate in 7 steps, involving chlorination, protection of the two phenolic groups, and hydrolysis to the benzoic acid. Formation of the dianion and quenching with a Weinreb amide results in acylation of the toluene methyl group that is followed by amide formation and ring closing metathesis to form the macrocyclic lactam. Final deprotection of the phenolic groups gives the desired macrolactams whose binding to the N-terminal domain of yeast Hsp90 was studied by isothermal titration calorimetry and protein X-ray crystallography.


==Function==
Synthesis of macrolactam analogues of radicicol and their binding to heat shock protein Hsp90.,Dutton BL, Kitson RR, Parry-Morris S, Roe SM, Prodromou C, Moody CJ Org Biomol Chem. 2014 Jan 17. PMID:24435512<ref>PMID:24435512</ref>
[[http://www.uniprot.org/uniprot/HSP82_YEAST HSP82_YEAST]] Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. The nucleotide-free form of the dimer is found in an open conformation in which the N-termini are not dimerized and the complex is ready for client protein binding. Binding of ATP induces large conformational changes, resulting in the formation of a ring-like closed structure in which the N-terminal domains associate intramolecularly with the middle domain and also dimerize with each other, stimulating their intrinsic ATPase activity and acting as a clamp on the substrate. Finally, ATP hydrolysis results in the release of the substrate. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Required for growth at high temperatures.<ref>PMID:17114002</ref>


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4ce1]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CE1 OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:024435512</ref><references group="xtra"/><references/>
__TOC__
[[Category: Parry-Morris, S.]]
</StructureSection>
[[Category: Prodromou, C.]]
[[Category: Atcc 18824]]
[[Category: Roe, S M.]]
[[Category: Parry-Morris, S]]
[[Category: Prodromou, C]]
[[Category: Roe, S M]]
[[Category: Chaperone]]
[[Category: Chaperone]]

Revision as of 21:40, 21 December 2014

Hsp90 N-terminal domain bound to macrolactam analogues of radicicol.Hsp90 N-terminal domain bound to macrolactam analogues of radicicol.

Structural highlights

4ce1 is a 1 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

A series of macrolactam analogues of the naturally occurring resorcylic acid lactone radicicol have been synthesised from methyl orsellinate in 7 steps, involving chlorination, protection of the two phenolic groups, and hydrolysis to the benzoic acid. Formation of the dianion and quenching with a Weinreb amide results in acylation of the toluene methyl group that is followed by amide formation and ring closing metathesis to form the macrocyclic lactam. Final deprotection of the phenolic groups gives the desired macrolactams whose binding to the N-terminal domain of yeast Hsp90 was studied by isothermal titration calorimetry and protein X-ray crystallography.

Synthesis of macrolactam analogues of radicicol and their binding to heat shock protein Hsp90.,Dutton BL, Kitson RR, Parry-Morris S, Roe SM, Prodromou C, Moody CJ Org Biomol Chem. 2014 Jan 17. PMID:24435512[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Dutton BL, Kitson RR, Parry-Morris S, Roe SM, Prodromou C, Moody CJ. Synthesis of macrolactam analogues of radicicol and their binding to heat shock protein Hsp90. Org Biomol Chem. 2014 Jan 17. PMID:24435512 doi:http://dx.doi.org/10.1039/c3ob42211a

4ce1, resolution 2.01Å

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