4ik3: Difference between revisions
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==High resolution structure of GCaMP3 at pH 8.5== | |||
<StructureSection load='4ik3' size='340' side='right' caption='[[4ik3]], [[Resolution|resolution]] 2.01Å' scene=''> | |||
{ | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ik3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IK3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IK3 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ik1|4ik1]], [[4ik4|4ik4]], [[4ik5|4ik5]], [[4ik8|4ik8]], [[4ik9|4ik9]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ik3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ik3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ik3 RCSB], [http://www.ebi.ac.uk/pdbsum/4ik3 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Genetically encoded Ca(2+) indicators (GECI) are important for the measurement of Ca(2+) in vivo. GCaMP2, a widely-used GECI, has recently been iteratively improved. Among the improved variants, GCaMP3 exhibits significantly better fluorescent intensity. In this study, we developed a new GECI called GCaMPJ and determined the crystal structures of GCaMP3 and GCaMPJ. GCaMPJ has a 1.5-fold increase in fluorescence and 1.3-fold increase in calcium affinity over GCaMP3. Upon Ca(2+) binding, GCaMP3 exhibits both monomeric and dimeric forms. The structural superposition of these two forms reveals the role of Arg-376 in improving monomer performance. However, GCaMPJ seldom forms dimers under conditions similar to GCaMP3. St ructural and mutagenesis studies on Tyr-380 confirmed its importance in blocking the cpEGFP beta-barrel holes. Our study proposes an efficient tool for mapping Ca(2+) signals in intact organs to facilitate the further improvement of GCaMP sensors. | |||
Structural insight into enhanced calcium indicator GCaMP3 and GCaMPJ to promote further improvement.,Chen Y, Song X, Ye S, Miao L, Zhu Y, Zhang RG, Ji G Protein Cell. 2013 Apr;4(4):299-309. doi: 10.1007/s13238-013-2103-4. Epub 2013, Apr 3. PMID:23549615<ref>PMID:23549615</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Chen, Y | |||
[[Category: Ji, G | ==See Also== | ||
[[Category: Miao, L | *[[Green Fluorescent Protein|Green Fluorescent Protein]] | ||
[[Category: Song, X | == References == | ||
[[Category: Zhu, Y | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Aeqvi]] | |||
[[Category: Chen, Y]] | |||
[[Category: Ji, G]] | |||
[[Category: Miao, L]] | |||
[[Category: Song, X]] | |||
[[Category: Zhu, Y]] | |||
[[Category: Beta barrel]] | [[Category: Beta barrel]] | ||
[[Category: Calcium indicator]] | [[Category: Calcium indicator]] | ||