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{{STRUCTURE_4lhx| PDB=4lhx | SCENE= }}
==Crystal structure of nucleotide-free Rab8:Rabin8==
===Crystal structure of nucleotide-free Rab8:Rabin8===
<StructureSection load='4lhx' size='340' side='right' caption='[[4lhx]], [[Resolution|resolution]] 3.05&Aring;' scene=''>
{{ABSTRACT_PUBMED_24072714}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4lhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LHX FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lhv|4lhv]], [[4lhw|4lhw]], [[4lhy|4lhy]], [[4lhz|4lhz]], [[4li0|4li0]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RAB8A, MEL, RAB8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), RAB3IP, RABIN8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lhx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lhx RCSB], [http://www.ebi.ac.uk/pdbsum/4lhx PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Small G-proteins (G) of the Ras-superfamily control the temporal and spatial coordination of intracellular signaling networks by acting as molecular on/off switches. Guanine nucleotide exchange factors (GEFs) regulate the activation of these G-proteins through catalytic replace-ment of guanosine diphosphate (GDP) by gua-nosine triphosphate (GTP). During nucleotide exchange, three distinct substrate-enzyme complexes occur: A ternary complex with GDP at the start of reaction (G:GDP:GEF), an intermediary nucleotide-free binary complex (G:GEF), and a ternary GTP-complex after productive G-protein activation (G:GTP:GEF). Here we show structural snapshots of the full nucleotide-exchange reaction sequence together with the G-protein substrates and products using Rabin8/GRAB (GEF) and Rab8 (G-protein) as a model system. Together with a thorough enzymatic characterization, our data provide a detailed view into the mechanism of Rabin8/GRAB mediated nucleotide exchange.


==Function==
Intermediates in the Guanine Nucleotide Exchange Reaction of Rab8 Catalyzed by Rabin8/GRAB.,Guo Z, Hou X, Goody RS, Itzen A J Biol Chem. 2013 Sep 26. PMID:24072714<ref>PMID:24072714</ref>
[[http://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN]] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>  [[http://www.uniprot.org/uniprot/RAB3I_HUMAN RAB3I_HUMAN]] Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.<ref>PMID:12221131</ref> <ref>PMID:20937701</ref> <ref>PMID:20890297</ref>


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4lhx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LHX OCA].
</div>
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:024072714</ref><references group="xtra"/><references/>
__TOC__
</StructureSection>
[[Category: Human]]
[[Category: Human]]
[[Category: Goody, R S.]]
[[Category: Goody, R S]]
[[Category: Guo, Z.]]
[[Category: Guo, Z]]
[[Category: Hou, X M.]]
[[Category: Hou, X M]]
[[Category: Itzen, A.]]
[[Category: Itzen, A]]
[[Category: Guanine nucleotide exchange factor]]
[[Category: Guanine nucleotide exchange factor]]
[[Category: Protein transport]]
[[Category: Protein transport]]
[[Category: Small gtpase]]
[[Category: Small gtpase]]

Revision as of 20:29, 21 December 2014

Crystal structure of nucleotide-free Rab8:Rabin8Crystal structure of nucleotide-free Rab8:Rabin8

Structural highlights

4lhx is a 6 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:RAB8A, MEL, RAB8 (HUMAN), RAB3IP, RABIN8 (HUMAN)
Resources:FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

Small G-proteins (G) of the Ras-superfamily control the temporal and spatial coordination of intracellular signaling networks by acting as molecular on/off switches. Guanine nucleotide exchange factors (GEFs) regulate the activation of these G-proteins through catalytic replace-ment of guanosine diphosphate (GDP) by gua-nosine triphosphate (GTP). During nucleotide exchange, three distinct substrate-enzyme complexes occur: A ternary complex with GDP at the start of reaction (G:GDP:GEF), an intermediary nucleotide-free binary complex (G:GEF), and a ternary GTP-complex after productive G-protein activation (G:GTP:GEF). Here we show structural snapshots of the full nucleotide-exchange reaction sequence together with the G-protein substrates and products using Rabin8/GRAB (GEF) and Rab8 (G-protein) as a model system. Together with a thorough enzymatic characterization, our data provide a detailed view into the mechanism of Rabin8/GRAB mediated nucleotide exchange.

Intermediates in the Guanine Nucleotide Exchange Reaction of Rab8 Catalyzed by Rabin8/GRAB.,Guo Z, Hou X, Goody RS, Itzen A J Biol Chem. 2013 Sep 26. PMID:24072714[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Guo Z, Hou X, Goody RS, Itzen A. Intermediates in the Guanine Nucleotide Exchange Reaction of Rab8 Catalyzed by Rabin8/GRAB. J Biol Chem. 2013 Sep 26. PMID:24072714 doi:http://dx.doi.org/10.1074/jbc.M113.498329

4lhx, resolution 3.05Å

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