3wc0: Difference between revisions
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==Crystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with GTP== | |||
<StructureSection load='3wc0' size='340' side='right' caption='[[3wc0]], [[Resolution|resolution]] 3.03Å' scene=''> | |||
=== | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3wc0]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Canal Canal]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WC0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WC0 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wbz|3wbz]], [[3wc1|3wc1]], [[3wc2|3wc2]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CaO19.7063, CAWG_05412, orf19.7063, THG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=237561 CANAL])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wc0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wc0 RCSB], [http://www.ebi.ac.uk/pdbsum/3wc0 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Nucleotide polymerization proceeds in the forward (5'-3') direction. This tenet of the central dogma of molecular biology is found in diverse processes including transcription, reverse transcription, DNA replication, and even in lagging strand synthesis where reverse polymerization (3'-5') would present a "simpler" solution. Interestingly, reverse (3'-5') nucleotide addition is catalyzed by the tRNA maturation enzyme tRNA(His) guanylyltransferase, a structural homolog of canonical forward polymerases. We present a Candida albicans tRNA(His) guanylyltransferase-tRNA(His) complex structure that reveals the structural basis of reverse polymerization. The directionality of nucleotide polymerization is determined by the orientation of approach of the nucleotide substrate. The tRNA substrate enters the enzyme's active site from the opposite direction (180 degrees flip) compared with similar nucleotide substrates of canonical 5'-3' polymerases, and the finger domains are on opposing sides of the core palm domain. Structural, biochemical, and phylogenetic data indicate that reverse polymerization appeared early in evolution and resembles a mirror image of the forward process. | |||
Structural basis of reverse nucleotide polymerization.,Nakamura A, Nemoto T, Heinemann IU, Yamashita K, Sonoda T, Komoda K, Tanaka I, Soll D, Yao M Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):20970-5. doi:, 10.1073/pnas.1321312111. Epub 2013 Dec 9. PMID:24324136<ref>PMID:24324136</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Nakamura, A | == References == | ||
[[Category: Nemoto, T | <references/> | ||
[[Category: Sonoda, T | __TOC__ | ||
[[Category: Tanaka, I | </StructureSection> | ||
[[Category: Yamashita, K | [[Category: Canal]] | ||
[[Category: Yao, M | [[Category: Nakamura, A]] | ||
[[Category: Nemoto, T]] | |||
[[Category: Sonoda, T]] | |||
[[Category: Tanaka, I]] | |||
[[Category: Yamashita, K]] | |||
[[Category: Yao, M]] | |||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 19:13, 21 December 2014
Crystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with GTPCrystal structure of C. albicans tRNA(His) guanylyltransferase (Thg1) with GTP
Structural highlights
Publication Abstract from PubMedNucleotide polymerization proceeds in the forward (5'-3') direction. This tenet of the central dogma of molecular biology is found in diverse processes including transcription, reverse transcription, DNA replication, and even in lagging strand synthesis where reverse polymerization (3'-5') would present a "simpler" solution. Interestingly, reverse (3'-5') nucleotide addition is catalyzed by the tRNA maturation enzyme tRNA(His) guanylyltransferase, a structural homolog of canonical forward polymerases. We present a Candida albicans tRNA(His) guanylyltransferase-tRNA(His) complex structure that reveals the structural basis of reverse polymerization. The directionality of nucleotide polymerization is determined by the orientation of approach of the nucleotide substrate. The tRNA substrate enters the enzyme's active site from the opposite direction (180 degrees flip) compared with similar nucleotide substrates of canonical 5'-3' polymerases, and the finger domains are on opposing sides of the core palm domain. Structural, biochemical, and phylogenetic data indicate that reverse polymerization appeared early in evolution and resembles a mirror image of the forward process. Structural basis of reverse nucleotide polymerization.,Nakamura A, Nemoto T, Heinemann IU, Yamashita K, Sonoda T, Komoda K, Tanaka I, Soll D, Yao M Proc Natl Acad Sci U S A. 2013 Dec 24;110(52):20970-5. doi:, 10.1073/pnas.1321312111. Epub 2013 Dec 9. PMID:24324136[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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