4mgm: Difference between revisions
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==Crystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly== | |||
<StructureSection load='4mgm' size='340' side='right' caption='[[4mgm]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4mgm]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MGM FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4mgn|4mgn]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mgm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4mgm RCSB], [http://www.ebi.ac.uk/pdbsum/4mgm PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
T box riboswitches are cis-acting RNA elements that bind to tRNA and sense its aminoacylation state to influence gene expression. Here, we present the 3.2 A resolution X-ray crystal structures of the T box Stem I-tRNA complex and tRNA, in isolation. T box Stem I forms an arched conformation with extensive intermolecular contacts to two key points of tRNA, the anticodon and D/T-loops. Free and complexed tRNA exist in significantly different conformations, with the contacts stabilizing flexible D/T-loops and a rearrangement of the D-loop. Using a designed T box RNA/tRNA system, we demonstrate that the T box riboswitch monitors the length and orientation of two essential contacts. Length or orientation mismatches engineered into the T box riboswitch and tRNA disrupt the complex, whereas simultaneous insertion of full helical turns realigns the interfaces and restores interaction between artificially elongated T box riboswitch and tRNA molecules. | |||
Structural Determinants for Geometry and Information Decoding of tRNA by T Box Leader RNA.,Grigg JC, Ke A Structure. 2013 Oct 1. pii: S0969-2126(13)00346-8. doi:, 10.1016/j.str.2013.09.001. PMID:24095061<ref>PMID:24095061</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Grigg, J C | == References == | ||
[[Category: Ke, A | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Grigg, J C]] | |||
[[Category: Ke, A]] | |||
[[Category: Base stacking]] | [[Category: Base stacking]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
Revision as of 18:38, 21 December 2014
Crystal structure of the in vitro transcribed G. kaustophilus tRNA-GlyCrystal structure of the in vitro transcribed G. kaustophilus tRNA-Gly
Structural highlights
Publication Abstract from PubMedT box riboswitches are cis-acting RNA elements that bind to tRNA and sense its aminoacylation state to influence gene expression. Here, we present the 3.2 A resolution X-ray crystal structures of the T box Stem I-tRNA complex and tRNA, in isolation. T box Stem I forms an arched conformation with extensive intermolecular contacts to two key points of tRNA, the anticodon and D/T-loops. Free and complexed tRNA exist in significantly different conformations, with the contacts stabilizing flexible D/T-loops and a rearrangement of the D-loop. Using a designed T box RNA/tRNA system, we demonstrate that the T box riboswitch monitors the length and orientation of two essential contacts. Length or orientation mismatches engineered into the T box riboswitch and tRNA disrupt the complex, whereas simultaneous insertion of full helical turns realigns the interfaces and restores interaction between artificially elongated T box riboswitch and tRNA molecules. Structural Determinants for Geometry and Information Decoding of tRNA by T Box Leader RNA.,Grigg JC, Ke A Structure. 2013 Oct 1. pii: S0969-2126(13)00346-8. doi:, 10.1016/j.str.2013.09.001. PMID:24095061[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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