4llr: Difference between revisions
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==Tryparedoxin peroxidase (TXNPX) from trypanosoma cruzi in the reduced state== | |||
===Tryparedoxin peroxidase ( | <StructureSection load='4llr' size='340' side='right' caption='[[4llr]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4llr]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1uul 1uul]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LLR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LLR FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tryp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5693 Trypanosoma cruzi])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4llr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4llr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4llr RCSB], [http://www.ebi.ac.uk/pdbsum/4llr PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Tryparedoxin peroxidase from Trypanosoma cruzi (TcTXNPx) belongs to the family of typical 2-Cys peroxiredoxins. These enzymes function as antioxidants through their peroxidase and peroxynitrite reductase activities. In T. cruzi, as in all trypanosomatids, this enzyme is the final electron acceptor of a unique system for detoxifying hydroperoxides, constituting a relevant target for drug design. We have determined the crystal structure of TcTXPNx in the reduced active state. The structure comprises 10 subunits in the asymmetric unit, associated to form a decamer of toroidal shape obeying 52 (D5) point group symmetry. We have analyzed the structure of TcTXNPx by comparing it with other structures of typical 2-Cys peroxiredoxins in both redox states, and have identified key residues in the structural rearrangement taking place in the enzymatic cycle. This is the first report of the structure of an active peroxiredoxin that has peroxidase and peroxynitrite reductase activity, and it is noteworthy that it is from a human parasite. This knowledge is of interest for further understanding peroxide metabolism in these parasites, and in the design of new trypanosomatidal drugs against Chagas disease. | |||
Crystal structure of the tryparedoxin peroxidase from the human parasite Trypanosoma cruzi.,Pineyro MD, Pizarro JC, Lema F, Pritsch O, Cayota A, Bentley GA, Robello C J Struct Biol. 2005 Apr;150(1):11-22. PMID:15797726<ref>PMID:15797726</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Trypanosoma cruzi]] | [[Category: Trypanosoma cruzi]] | ||
[[Category: Bentley, G A | [[Category: Bentley, G A]] | ||
[[Category: Cayota, A | [[Category: Cayota, A]] | ||
[[Category: Lema, F | [[Category: Lema, F]] | ||
[[Category: Pineyro, M D | [[Category: Pineyro, M D]] | ||
[[Category: Pizarro, J C | [[Category: Pizarro, J C]] | ||
[[Category: Pritsch, O | [[Category: Pritsch, O]] | ||
[[Category: Robello, C | [[Category: Robello, C]] | ||
[[Category: Oxidoreductase]] | [[Category: Oxidoreductase]] | ||
[[Category: Peroxidase]] | [[Category: Peroxidase]] | ||
[[Category: Peroxiredoxin]] | [[Category: Peroxiredoxin]] | ||
Revision as of 17:45, 21 December 2014
Tryparedoxin peroxidase (TXNPX) from trypanosoma cruzi in the reduced stateTryparedoxin peroxidase (TXNPX) from trypanosoma cruzi in the reduced state
Structural highlights
Publication Abstract from PubMedTryparedoxin peroxidase from Trypanosoma cruzi (TcTXNPx) belongs to the family of typical 2-Cys peroxiredoxins. These enzymes function as antioxidants through their peroxidase and peroxynitrite reductase activities. In T. cruzi, as in all trypanosomatids, this enzyme is the final electron acceptor of a unique system for detoxifying hydroperoxides, constituting a relevant target for drug design. We have determined the crystal structure of TcTXPNx in the reduced active state. The structure comprises 10 subunits in the asymmetric unit, associated to form a decamer of toroidal shape obeying 52 (D5) point group symmetry. We have analyzed the structure of TcTXNPx by comparing it with other structures of typical 2-Cys peroxiredoxins in both redox states, and have identified key residues in the structural rearrangement taking place in the enzymatic cycle. This is the first report of the structure of an active peroxiredoxin that has peroxidase and peroxynitrite reductase activity, and it is noteworthy that it is from a human parasite. This knowledge is of interest for further understanding peroxide metabolism in these parasites, and in the design of new trypanosomatidal drugs against Chagas disease. Crystal structure of the tryparedoxin peroxidase from the human parasite Trypanosoma cruzi.,Pineyro MD, Pizarro JC, Lema F, Pritsch O, Cayota A, Bentley GA, Robello C J Struct Biol. 2005 Apr;150(1):11-22. PMID:15797726[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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