1s7l: Difference between revisions
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[[Image:1s7l.gif|left|200px]] | [[Image:1s7l.gif|left|200px]] | ||
'''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase in complex with Coenzyme A (CoA-Cys134 Disulfide)''' | {{Structure | ||
|PDB= 1s7l |SIZE=350|CAPTION= <scene name='initialview01'>1s7l</scene>, resolution 2.3Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=COA:COENZYME A'>COA</scene> | |||
|ACTIVITY= | |||
|GENE= RimL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 Salmonella typhimurium LT2]) | |||
}} | |||
'''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase in complex with Coenzyme A (CoA-Cys134 Disulfide)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1S7L is a [ | 1S7L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7L OCA]. | ||
==Reference== | ==Reference== | ||
A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:[http:// | A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15817456 15817456] | ||
[[Category: Salmonella typhimurium lt2]] | [[Category: Salmonella typhimurium lt2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: acetyltransferase]] | [[Category: acetyltransferase]] | ||
[[Category: alpha-n-protein acetyltransferase]] | [[Category: alpha-n-protein acetyltransferase]] | ||
[[Category: coenzyme | [[Category: coenzyme some]] | ||
[[Category: gnat]] | [[Category: gnat]] | ||
[[Category: l7/l12]] | [[Category: l7/l12]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:33 2008'' | ||
Revision as of 15:01, 20 March 2008
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| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | RimL (Salmonella typhimurium LT2) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase in complex with Coenzyme A (CoA-Cys134 Disulfide)
OverviewOverview
RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation.
About this StructureAbout this Structure
1S7L is a Single protein structure of sequence from Salmonella typhimurium lt2. Full crystallographic information is available from OCA.
ReferenceReference
A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:15817456
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