1s68: Difference between revisions

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Revision as of 15:01, 20 March 2008

File:1s68.jpg

, resolution 1.90Å

Ligands:

Gene:

Y10A, 24.1 (Bacteriophage T4)

Coordinates:

save as pdb, mmCIF, xml

Structure and Mechanism of RNA Ligase

OverviewOverview

T4 RNA ligase 2 (Rnl2) exemplifies an RNA ligase family that includes the RNA editing ligases (RELs) of Trypanosoma and Leishmania. The Rnl2/REL enzymes are defined by essential signature residues and a unique C-terminal domain, which we show is essential for sealing of 3'-OH and 5'-PO4 RNA ends by Rnl2, but not for ligase adenylation or phosphodiester bond formation at a preadenylated AppRNA end. The N-terminal segment Rnl2(1-249) of the 334 aa Rnl2 protein comprises an autonomous adenylyltransferase/AppRNA ligase domain. We report the 1.9 A crystal structure of the ligase domain with AMP bound at the active site, which reveals a shared fold, catalytic mechanism, and evolutionary history for RNA ligases, DNA ligases, and mRNA capping enzymes.

About this StructureAbout this Structure

1S68 is a Single protein structure of sequence from Bacteriophage t4. Full crystallographic information is available from OCA.

ReferenceReference

Structure and mechanism of RNA ligase., Ho CK, Wang LK, Lima CD, Shuman S, Structure. 2004 Feb;12(2):327-39. PMID:14962393

Page seeded by OCA on Thu Mar 20 14:01:02 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1s68.jpg|left|200px]]<br /><applet load="1s68" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1s68.jpg|left|200px]]
-caption="1s68, resolution 1.90&Aring;" />
-'''Structure and Mechanism of RNA Ligase'''<br />
+ {{Structure
+|PDB= 1s68 |SIZE=350|CAPTION= <scene name='initialview01'>1s68</scene>, resolution 1.90&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=AMP:ADENOSINE MONOPHOSPHATE'>AMP</scene>
+|ACTIVITY=
+|GENE= Y10A, 24.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Bacteriophage T4])
+}}
+'''Structure and Mechanism of RNA Ligase'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-S68 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=AMP:'>AMP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S68 OCA].
+S68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S68 OCA].
 ==Reference==
-Structure and mechanism of RNA ligase., Ho CK, Wang LK, Lima CD, Shuman S, Structure. 2004 Feb;12(2):327-39. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14962393 14962393]
+Structure and mechanism of RNA ligase., Ho CK, Wang LK, Lima CD, Shuman S, Structure. 2004 Feb;12(2):327-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962393 14962393]
 [[Category: Bacteriophage t4]]
 [[Category: Single protein]]
@@ Line 22: / Line 31: @@
 [[Category: t4]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:58:23 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:02 2008''