1s3t: Difference between revisions

← Older edit Newer edit →

Revision as of 15:00, 20 March 2008

File:1s3t.gif

, resolution 2.10Å

Ligands:

, and

Activity:

Urease, with EC number 3.5.1.5

Coordinates:

save as pdb, mmCIF, xml

BORATE INHIBITED BACILLUS PASTEURII UREASE CRYSTAL STRUCTURE

OverviewOverview

The structure of the complex of urease, a Ni-containing metalloenzyme, with boric acid was determined at 2.10 A resolution. The complex shows the unprecedented binding mode of the competitive inhibitor to the dinuclear metal center, with the B(OH)3 molecule bridging the Ni ions and leaving in place the bridging hydroxide. Boric acid can be considered a substrate analogue of urea, and the structure supports the proposal that the Ni-bridging hydroxide acts as the nucleophile in the enzymatic process of urea hydrolysis.

About this StructureAbout this Structure

1S3T is a Protein complex structure of sequences from Sporosarcina pasteurii. Full crystallographic information is available from OCA.

ReferenceReference

Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism., Benini S, Rypniewski WR, Wilson KS, Mangani S, Ciurli S, J Am Chem Soc. 2004 Mar 31;126(12):3714-5. PMID:15038715

Page seeded by OCA on Thu Mar 20 14:00:03 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1s3t.gif|left|200px]]<br /><applet load="1s3t" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1s3t.gif|left|200px]]
-caption="1s3t, resolution 2.10&Aring;" />
-'''BORATE INHIBITED BACILLUS PASTEURII UREASE CRYSTAL STRUCTURE'''<br />
+ {{Structure
+|PDB= 1s3t |SIZE=350|CAPTION= <scene name='initialview01'>1s3t</scene>, resolution 2.10&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=BO3:BORIC ACID'>BO3</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5]
+|GENE=
+}}
+'''BORATE INHIBITED BACILLUS PASTEURII UREASE CRYSTAL STRUCTURE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-S3T is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii] with <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BO3:'>BO3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Urease Urease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S3T OCA].
+S3T is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sporosarcina_pasteurii Sporosarcina pasteurii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S3T OCA].
 ==Reference==
-Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism., Benini S, Rypniewski WR, Wilson KS, Mangani S, Ciurli S, J Am Chem Soc. 2004 Mar 31;126(12):3714-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15038715 15038715]
+Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism., Benini S, Rypniewski WR, Wilson KS, Mangani S, Ciurli S, J Am Chem Soc. 2004 Mar 31;126(12):3714-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15038715 15038715]
 [[Category: Protein complex]]
 [[Category: Sporosarcina pasteurii]]
@@ Line 28: / Line 37: @@
 [[Category: urease]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:57:36 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:00:03 2008''