2c7n: Difference between revisions
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==Overview== | ==Overview== | ||
The interaction between ubiquitinated proteins and intracellular proteins, harboring ubiquitin binding domains (UBDs) is critical to a multitude of, cellular processes. Here, we report that Rabex-5, a guanine nucleotide, exchange factor for Rab5, binds to Ub through two independent UBDs. These, UBDs determine a number of properties of Rabex-5, including its coupled, monoubiquitination and interaction in vivo with ubiquitinated EGFRs., Structural and biochemical characterization of the UBDs of Rabex-5, revealed that one of them (MIU, motif interacting with ubiquitin) binds to, Ub with modes superimposable to those of the UIM (ubiquitin-interacting, motif):Ub interaction, although in the opposite orientation. The other, UBD, RUZ (Rabex-5 ubiquitin binding zinc finger) binds to a surface of .. | The interaction between ubiquitinated proteins and intracellular proteins, harboring ubiquitin binding domains (UBDs) is critical to a multitude of, cellular processes. Here, we report that Rabex-5, a guanine nucleotide, exchange factor for Rab5, binds to Ub through two independent UBDs. These, UBDs determine a number of properties of Rabex-5, including its coupled, monoubiquitination and interaction in vivo with ubiquitinated EGFRs., Structural and biochemical characterization of the UBDs of Rabex-5, revealed that one of them (MIU, motif interacting with ubiquitin) binds to, Ub with modes superimposable to those of the UIM (ubiquitin-interacting, motif):Ub interaction, although in the opposite orientation. The other, UBD, RUZ (Rabex-5 ubiquitin binding zinc finger) binds to a surface of Ub, centered on Asp58(Ub) and distinct from the "canonical" Ile44(Ub)-based, surface. The two binding surfaces allow Ub to interact simultaneously with, different UBDs, thus opening new perspectives in Ub-mediated signaling. | ||
==About this Structure== | ==About this Structure== | ||
2C7N is a | 2C7N is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2C7N OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: ubl conjugation]] | [[Category: ubl conjugation]] | ||
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Revision as of 13:38, 5 November 2007
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HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN
OverviewOverview
The interaction between ubiquitinated proteins and intracellular proteins, harboring ubiquitin binding domains (UBDs) is critical to a multitude of, cellular processes. Here, we report that Rabex-5, a guanine nucleotide, exchange factor for Rab5, binds to Ub through two independent UBDs. These, UBDs determine a number of properties of Rabex-5, including its coupled, monoubiquitination and interaction in vivo with ubiquitinated EGFRs., Structural and biochemical characterization of the UBDs of Rabex-5, revealed that one of them (MIU, motif interacting with ubiquitin) binds to, Ub with modes superimposable to those of the UIM (ubiquitin-interacting, motif):Ub interaction, although in the opposite orientation. The other, UBD, RUZ (Rabex-5 ubiquitin binding zinc finger) binds to a surface of Ub, centered on Asp58(Ub) and distinct from the "canonical" Ile44(Ub)-based, surface. The two binding surfaces allow Ub to interact simultaneously with, different UBDs, thus opening new perspectives in Ub-mediated signaling.
About this StructureAbout this Structure
2C7N is a Protein complex structure of sequences from Homo sapiens with ZN as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin., Penengo L, Mapelli M, Murachelli AG, Confalonieri S, Magri L, Musacchio A, Di Fiore PP, Polo S, Schneider TR, Cell. 2006 Mar 24;124(6):1183-95. Epub 2006 Feb 23. PMID:16499958
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