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{{STRUCTURE_4j89|  PDB=4j89  |  SCENE=  }}
==Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence==
===Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence===
<StructureSection load='4j89' size='340' side='right' caption='[[4j89]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
{{ABSTRACT_PUBMED_23620472}}
== Structural highlights ==
<table><tr><td colspan='2'>[[4j89]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J89 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4J89 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CQ2:{(4Z)-4-(4-AMINOBENZYLIDENE)-2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CQ2</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j88|4j88]], [[4j8a|4j8a]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4j89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4j89 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4j89 RCSB], [http://www.ebi.ac.uk/pdbsum/4j89 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.


==Function==
Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.,Reddington SC, Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD Angew Chem Int Ed Engl. 2013 Apr 25. doi: 10.1002/anie.201301490. PMID:23620472<ref>PMID:23620472</ref>
[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[4j89]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4J89 OCA].
</div>


==Reference==
==See Also==
<ref group="xtra">PMID:023620472</ref><references group="xtra"/><references/>
*[[Green Fluorescent Protein|Green Fluorescent Protein]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
[[Category: Jones, D D.]]
[[Category: Jones, D D]]
[[Category: Reddington, S C.]]
[[Category: Reddington, S C]]
[[Category: Rizkallah, P J.]]
[[Category: Rizkallah, P J]]
[[Category: Tippmann, E M.]]
[[Category: Tippmann, E M]]
[[Category: Beta-barrel]]
[[Category: Beta-barrel]]
[[Category: Chromophore by cyclisation]]
[[Category: Chromophore by cyclisation]]

Revision as of 15:04, 21 December 2014

Different photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescenceDifferent photochemical events of a genetically encoded aryl azide define and modulate GFP fluorescence

Structural highlights

4j89 is a 2 chain structure with sequence from Aequorea victoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, ,
NonStd Res:
Gene:GFP (Aequorea victoria)
Resources:FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.

Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence.,Reddington SC, Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD Angew Chem Int Ed Engl. 2013 Apr 25. doi: 10.1002/anie.201301490. PMID:23620472[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Reddington SC, Rizkallah PJ, Watson PD, Pearson R, Tippmann EM, Jones DD. Different Photochemical Events of a Genetically Encoded Phenyl Azide Define and Modulate GFP Fluorescence. Angew Chem Int Ed Engl. 2013 Apr 25. doi: 10.1002/anie.201301490. PMID:23620472 doi:10.1002/anie.201301490

4j89, resolution 2.10Å

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