1s00: Difference between revisions

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Revision as of 14:58, 20 March 2008

File:1s00.gif

, resolution 2.60Å

Ligands:

, , , , , and

Gene:

PUFL (Rhodobacter sphaeroides), PUFM (Rhodobacter sphaeroides), PUHA (Rhodobacter sphaeroides)

Coordinates:

save as pdb, mmCIF, xml

PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG M233 REPLACED WITH CYS IN THE CHARGE-SEPARATED D+QAQB- STATE

OverviewOverview

In the photosynthetic reaction center (RC) from Rhodobacter sphaeroides, the reduction of a bound quinone molecule Q(B) is coupled with proton uptake. When Asp-L213 is replaced by Asn, proton transfer is inhibited. Proton transfer was restored by two second-site revertant mutations, Arg-M233-->Cys and Arg-H177-->His. Kinetic effects of Cd(2+) on proton transfer showed that the entry point in revertant RCs to be the same as in the native RC. The structures of the parental and two revertant RCs were determined at resolutions of 2.10, 1.80, and 2.75 A. From the structures, we were able to delineate alternate proton transfer pathways in the revertants. The main changes occur near Glu-H173, which allow it to substitute for the missing Asp-L213. The electrostatic changes near Glu-H173 cause it to be a good proton donor and acceptor, and the structural changes create a cavity which accommodates water molecules that connect Glu-H173 to other proton transfer components.

About this StructureAbout this Structure

1S00 is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.

ReferenceReference

X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways., Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G, Structure. 2004 Apr;12(4):703-15. PMID:15062092

Page seeded by OCA on Thu Mar 20 13:58:36 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1s00.gif|left|200px]]<br /><applet load="1s00" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1s00.gif|left|200px]]
-caption="1s00, resolution 2.60&Aring;" />
-'''PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG M233 REPLACED WITH CYS IN THE CHARGE-SEPARATED D+QAQB- STATE'''<br />
+ {{Structure
+|PDB= 1s00 |SIZE=350|CAPTION= <scene name='initialview01'>1s00</scene>, resolution 2.60&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=BPB:BACTERIOPHEOPHYTIN+B'>BPB</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene>, <scene name='pdbligand=SPO:SPHEROIDENE'>SPO</scene> and <scene name='pdbligand=LDA:LAURYL DIMETHYLAMINE-N-OXIDE'>LDA</scene>
+|ACTIVITY=
+|GENE= PUFL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), PUFM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), PUHA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides])
+}}
+'''PHOTOSYNTHETIC REACTION CENTER DOUBLE MUTANT FROM RHODOBACTER SPHAEROIDES WITH ASP L213 REPLACED WITH ASN AND ARG M233 REPLACED WITH CYS IN THE CHARGE-SEPARATED D+QAQB- STATE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-S00 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides] with <scene name='pdbligand=FE2:'>FE2</scene>, <scene name='pdbligand=BCL:'>BCL</scene>, <scene name='pdbligand=BPH:'>BPH</scene>, <scene name='pdbligand=BPB:'>BPB</scene>, <scene name='pdbligand=U10:'>U10</scene>, <scene name='pdbligand=SPO:'>SPO</scene> and <scene name='pdbligand=LDA:'>LDA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S00 OCA].
+S00 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S00 OCA].
 ==Reference==
-X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways., Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G, Structure. 2004 Apr;12(4):703-15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15062092 15062092]
+X-Ray structure determination of three mutants of the bacterial photosynthetic reaction centers from Rb. sphaeroides; altered proton transfer pathways., Xu Q, Axelrod HL, Abresch EC, Paddock ML, Okamura MY, Feher G, Structure. 2004 Apr;12(4):703-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15062092 15062092]
 [[Category: Protein complex]]
 [[Category: Rhodobacter sphaeroides]]
@@ Line 30: / Line 39: @@
 [[Category: proton transfer pathway]]
 [[Category: revertant]]
-[[Category: rhodobacter sphaeroides]]
+[[Category: rhodobacter sphaeroide]]
 [[Category: x-ray crystallography]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:31 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:58:36 2008''