1ry6: Difference between revisions
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'''Crystal Structure of Internal Kinesin Motor Domain''' | {{Structure | ||
|PDB= 1ry6 |SIZE=350|CAPTION= <scene name='initialview01'>1ry6</scene>, resolution 1.60Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= PFL2165W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5833 Plasmodium falciparum]) | |||
}} | |||
'''Crystal Structure of Internal Kinesin Motor Domain''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1RY6 is a [ | 1RY6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RY6 OCA]. | ||
==Reference== | ==Reference== | ||
Structure of a kinesin microtubule depolymerization machine., Shipley K, Hekmat-Nejad M, Turner J, Moores C, Anderson R, Milligan R, Sakowicz R, Fletterick R, EMBO J. 2004 Apr 7;23(7):1422-32. Epub 2004 Mar 18. PMID:[http:// | Structure of a kinesin microtubule depolymerization machine., Shipley K, Hekmat-Nejad M, Turner J, Moores C, Anderson R, Milligan R, Sakowicz R, Fletterick R, EMBO J. 2004 Apr 7;23(7):1422-32. Epub 2004 Mar 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15029249 15029249] | ||
[[Category: Plasmodium falciparum]] | [[Category: Plasmodium falciparum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: nucleotide-free]] | [[Category: nucleotide-free]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:57:49 2008'' | ||
Revision as of 14:57, 20 March 2008
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| , resolution 1.60Å | |||||||
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| Ligands: | |||||||
| Gene: | PFL2165W (Plasmodium falciparum) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Internal Kinesin Motor Domain
OverviewOverview
With their ability to depolymerize microtubules (MTs), KinI kinesins are the rogue members of the kinesin family. Here we present the 1.6 A crystal structure of a KinI motor core from Plasmodium falciparum, which is sufficient for depolymerization in vitro. Unlike all published kinesin structures to date, nucleotide is not present, and there are noticeable differences in loop regions L6 and L10 (the plus-end tip), L2 and L8 and in switch II (L11 and helix4); otherwise, the pKinI structure is very similar to previous kinesin structures. KinI-conserved amino acids were mutated to alanine, and studied for their effects on depolymerization and ATP hydrolysis. Notably, mutation of three residues in L2 appears to primarily affect depolymerization, rather than general MT binding or ATP hydrolysis. The results of this study confirm the suspected importance of loop 2 for KinI function, and provide evidence that KinI is specialized to hydrolyze ATP after initiating depolymerization.
About this StructureAbout this Structure
1RY6 is a Single protein structure of sequence from Plasmodium falciparum. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a kinesin microtubule depolymerization machine., Shipley K, Hekmat-Nejad M, Turner J, Moores C, Anderson R, Milligan R, Sakowicz R, Fletterick R, EMBO J. 2004 Apr 7;23(7):1422-32. Epub 2004 Mar 18. PMID:15029249
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