4dnu: Difference between revisions

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-{{STRUCTURE_4dnu|  PDB=4dnu  |  SCENE=  }}
+==Crystal structure of the W285A mutant of UVB-resistance protein UVR8==
-===Crystal structure of the W285A mutant of UVB-resistance protein UVR8===
+<StructureSection load='4dnu' size='340' side='right' caption='[[4dnu]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
-{{ABSTRACT_PUBMED_22388820}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4dnu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DNU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DNU FirstGlance]. <br>
+</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dnv|4dnv]], [[4dnw|4dnw]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UVR8, At5g63860, AT5G63860 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dnu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dnu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dnu RCSB], [http://www.ebi.ac.uk/pdbsum/4dnu PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Arabidopsis thaliana protein UVR8 is a photoreceptor for ultraviolet-B. Upon ultraviolet-B irradiation, UVR8 undergoes an immediate switch from homodimer to monomer, which triggers a signalling pathway for ultraviolet protection. The mechanism by which UVR8 senses ultraviolet-B remains largely unknown. Here we report the crystal structure of UVR8 at 1.8 A resolution, revealing a symmetric homodimer of seven-bladed beta-propeller that is devoid of any external cofactor as the chromophore. Arginine residues that stabilize the homodimeric interface, principally Arg 286 and Arg 338, make elaborate intramolecular cation-pi interactions with surrounding tryptophan amino acids. Two of these tryptophans, Trp 285 and Trp 233, collectively serve as the ultraviolet-B chromophore. Our structural and biochemical analyses identify the molecular mechanism for UVR8-mediated ultraviolet-B perception, in which ultraviolet-B radiation results in destabilization of the intramolecular cation-pi interactions, causing disruption of the critical intermolecular hydrogen bonds mediated by Arg 286 and Arg 338 and subsequent dissociation of the UVR8 homodimer.
-==About this Structure==
+Structural basis of ultraviolet-B perception by UVR8.,Wu D, Hu Q, Yan Z, Chen W, Yan C, Huang X, Zhang J, Yang P, Deng H, Wang J, Deng X, Shi Y Nature. 2012 Feb 29;484(7393):214-9. doi: 10.1038/nature10931. PMID:22388820<ref>PMID:22388820</ref>
-[[4dnu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DNU OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
 ==See Also==
 *[[Suggestions for new articles|Suggestions for new articles]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:022388820</ref><references group="xtra"/><references/>
+__TOC__
+</StructureSection>
 [[Category: Arabidopsis thaliana]]
-[[Category: Chen, W.]]
+[[Category: Chen, W]]
-[[Category: Hu, Q.]]
+[[Category: Hu, Q]]
-[[Category: Shi, Y.]]
+[[Category: Shi, Y]]
-[[Category: Wang, J.]]
+[[Category: Wang, J]]
-[[Category: Wu, D.]]
+[[Category: Wu, D]]
-[[Category: Yan, C.]]
+[[Category: Yan, C]]
-[[Category: Yan, Z.]]
+[[Category: Yan, Z]]
-[[Category: Zhang, J.]]
+[[Category: Zhang, J]]
 [[Category: Cop1]]
 [[Category: Gene regulation]]
 [[Category: Uv-b perception]]
 [[Category: Wd40 repeat]]