1rj9: Difference between revisions
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[[Image:1rj9.gif|left|200px]] | [[Image:1rj9.gif|left|200px]] | ||
'''Structure of the heterodimer of the conserved GTPase domains of the Signal Recognition Particle (Ffh) and Its Receptor (FtsY)''' | {{Structure | ||
|PDB= 1rj9 |SIZE=350|CAPTION= <scene name='initialview01'>1rj9</scene>, resolution 1.9Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER'>GCP</scene> | |||
|ACTIVITY= | |||
|GENE= FFH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus]) | |||
}} | |||
'''Structure of the heterodimer of the conserved GTPase domains of the Signal Recognition Particle (Ffh) and Its Receptor (FtsY)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1RJ9 is a [ | 1RJ9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJ9 OCA]. | ||
==Reference== | ==Reference== | ||
Substrate twinning activates the signal recognition particle and its receptor., Egea PF, Shan SO, Napetschnig J, Savage DF, Walter P, Stroud RM, Nature. 2004 Jan 15;427(6971):215-21. PMID:[http:// | Substrate twinning activates the signal recognition particle and its receptor., Egea PF, Shan SO, Napetschnig J, Savage DF, Walter P, Stroud RM, Nature. 2004 Jan 15;427(6971):215-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14724630 14724630] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Thermus aquaticus]] | [[Category: Thermus aquaticus]] | ||
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[[Category: srp-gtpase domain]] | [[Category: srp-gtpase domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:52:18 2008'' | ||
Revision as of 14:52, 20 March 2008
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| , resolution 1.9Å | |||||||
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| Ligands: | and | ||||||
| Gene: | FFH (Thermus aquaticus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the heterodimer of the conserved GTPase domains of the Signal Recognition Particle (Ffh) and Its Receptor (FtsY)
OverviewOverview
Signal sequences target proteins for secretion from cells or for integration into cell membranes. As nascent proteins emerge from the ribosome, signal sequences are recognized by the signal recognition particle (SRP), which subsequently associates with its receptor (SR). In this complex, the SRP and SR stimulate each other's GTPase activity, and GTP hydrolysis ensures unidirectional targeting of cargo through a translocation pore in the membrane. To define the mechanism of reciprocal activation, we determined the 1.9 A structure of the complex formed between these two GTPases. The two partners form a quasi-two-fold symmetrical heterodimer. Biochemical analysis supports the importance of the extensive interaction surface. Complex formation aligns the two GTP molecules in a symmetrical, composite active site, and the 3'OH groups are essential for association, reciprocal activation and catalysis. This unique circle of twinned interactions is severed twice on hydrolysis, leading to complex dissociation after cargo delivery.
About this StructureAbout this Structure
1RJ9 is a Protein complex structure of sequences from Thermus aquaticus. Full crystallographic information is available from OCA.
ReferenceReference
Substrate twinning activates the signal recognition particle and its receptor., Egea PF, Shan SO, Napetschnig J, Savage DF, Walter P, Stroud RM, Nature. 2004 Jan 15;427(6971):215-21. PMID:14724630
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