1rio: Difference between revisions
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[[Image:1rio.gif|left|200px]] | [[Image:1rio.gif|left|200px]] | ||
'''Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA''' | {{Structure | ||
|PDB= 1rio |SIZE=350|CAPTION= <scene name='initialview01'>1rio</scene>, resolution 2.30Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene> | |||
|ACTIVITY= | |||
|GENE= CI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda]) | |||
}} | |||
'''Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1RIO is a [ | 1RIO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda] and [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RIO OCA]. | ||
==Reference== | ==Reference== | ||
Structure of a ternary transcription activation complex., Jain D, Nickels BE, Sun L, Hochschild A, Darst SA, Mol Cell. 2004 Jan 16;13(1):45-53. PMID:[http:// | Structure of a ternary transcription activation complex., Jain D, Nickels BE, Sun L, Hochschild A, Darst SA, Mol Cell. 2004 Jan 16;13(1):45-53. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14731393 14731393] | ||
[[Category: Enterobacteria phage lambda]] | [[Category: Enterobacteria phage lambda]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: transcription activation]] | [[Category: transcription activation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:52:10 2008'' | ||
Revision as of 14:52, 20 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Gene: | CI (Enterobacteria phage lambda) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA
OverviewOverview
The cI protein of bacteriophage lambda (lambdacI) activates transcription by binding a DNA operator just upstream of the promoter and interacting with the RNA polymerase sigma subunit domain 4 (sigma(4)). We determined the crystal structure of the lambdacI/sigma(4)/DNA ternary complex at 2.3 A resolution. There are no conformational changes in either protein, which interact through an extremely small interface involving at most 6 amino acid residues. The interactions of the two proteins stabilize the binding of each protein to the DNA. The results provide insight into how activators can operate through a simple cooperative binding mechanism but affect different steps of the transcription initiation process.
About this StructureAbout this Structure
1RIO is a Protein complex structure of sequences from Enterobacteria phage lambda and Thermus aquaticus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a ternary transcription activation complex., Jain D, Nickels BE, Sun L, Hochschild A, Darst SA, Mol Cell. 2004 Jan 16;13(1):45-53. PMID:14731393
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