1ri4: Difference between revisions

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Revision as of 14:52, 20 March 2008

File:1ri4.jpg

, resolution 2.40Å

Ligands:

Gene:

ECU10_0380 (Encephalitozoon cuniculi)

Coordinates:

save as pdb, mmCIF, xml

Structure and mechanism of mRNA cap (guanine N-7) methyltransferase

OverviewOverview

A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.

About this StructureAbout this Structure

1RI4 is a Single protein structure of sequence from Encephalitozoon cuniculi. Full crystallographic information is available from OCA.

ReferenceReference

Structure and mechanism of mRNA cap (guanine-N7) methyltransferase., Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD, Mol Cell. 2004 Jan 16;13(1):77-89. PMID:14731396

Page seeded by OCA on Thu Mar 20 13:51:59 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1ri4.jpg|left|200px]]<br /><applet load="1ri4" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ri4.jpg|left|200px]]
-caption="1ri4, resolution 2.40&Aring;" />
-'''Structure and mechanism of mRNA cap (guanine N-7) methyltransferase'''<br />
+ {{Structure
+|PDB= 1ri4 |SIZE=350|CAPTION= <scene name='initialview01'>1ri4</scene>, resolution 2.40&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>
+|ACTIVITY=
+|GENE= ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])
+}}
+'''Structure and mechanism of mRNA cap (guanine N-7) methyltransferase'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-RI4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi] with <scene name='pdbligand=SAM:'>SAM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI4 OCA].
+RI4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI4 OCA].
 ==Reference==
-Structure and mechanism of mRNA cap (guanine-N7) methyltransferase., Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD, Mol Cell. 2004 Jan 16;13(1):77-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14731396 14731396]
+Structure and mechanism of mRNA cap (guanine-N7) methyltransferase., Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD, Mol Cell. 2004 Jan 16;13(1):77-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14731396 14731396]
 [[Category: Encephalitozoon cuniculi]]
 [[Category: Single protein]]
@@ Line 25: / Line 34: @@
 [[Category: rna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:15 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:51:59 2008''