1rh9: Difference between revisions
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[[Image:1rh9.gif|left|200px]] | [[Image:1rh9.gif|left|200px]] | ||
'''Family GH5 endo-beta-mannanase from Lycopersicon esculentum (tomato)''' | {{Structure | ||
|PDB= 1rh9 |SIZE=350|CAPTION= <scene name='initialview01'>1rh9</scene>, resolution 1.50Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Mannan_endo-1,4-beta-mannosidase Mannan endo-1,4-beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.78 3.2.1.78] | |||
|GENE= LeMAN4a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4081 Solanum lycopersicum]) | |||
}} | |||
'''Family GH5 endo-beta-mannanase from Lycopersicon esculentum (tomato)''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1RH9 is a [ | 1RH9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RH9 OCA]. | ||
==Reference== | ==Reference== | ||
Three-dimensional structure of (1,4)-beta-D-mannan mannanohydrolase from tomato fruit., Bourgault R, Oakley AJ, Bewley JD, Wilce MC, Protein Sci. 2005 May;14(5):1233-41. PMID:[http:// | Three-dimensional structure of (1,4)-beta-D-mannan mannanohydrolase from tomato fruit., Bourgault R, Oakley AJ, Bewley JD, Wilce MC, Protein Sci. 2005 May;14(5):1233-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15840830 15840830] | ||
[[Category: Mannan endo-1,4-beta-mannosidase]] | [[Category: Mannan endo-1,4-beta-mannosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: retaining]] | [[Category: retaining]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:51:36 2008'' | ||
Revision as of 14:51, 20 March 2008
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| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | LeMAN4a (Solanum lycopersicum) | ||||||
| Activity: | Mannan endo-1,4-beta-mannosidase, with EC number 3.2.1.78 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Family GH5 endo-beta-mannanase from Lycopersicon esculentum (tomato)
OverviewOverview
The three-dimensional crystal structure of tomato (Lycopersicon esculentum) beta-mannanase 4a (LeMAN4a) has been determined to 1.5 A resolution. The enzyme adopts the (beta/alpha)(8) fold common to the members of glycohydrolase family GH5. The structure is comparable with those of the homologous Trichoderma reesei and Thermomonospora fusca beta-mannanases: There is a conserved three-stranded beta-sheet located near the N terminus that stacks against the central beta-barrel at the end opposite the active site. Three noncanonical beta-helices surround the active site. Similar helices are found in T. reesei but not T. fusca beta-mannanase. By analogy with other beta-mannanases, the catalytic acid/base residue is E204 and the nucleophile residue is E318. The active site cleft of L. esculentum beta-mannanase most closely resembles that of the T. reesei isozyme. A model of substrate binding in LeMAN4a is proposed in which the mannosyl residue occupying the -1 subsite of the enzyme adopts the (1)S(5) skew-boat conformation.
About this StructureAbout this Structure
1RH9 is a Single protein structure of sequence from Solanum lycopersicum. Full crystallographic information is available from OCA.
ReferenceReference
Three-dimensional structure of (1,4)-beta-D-mannan mannanohydrolase from tomato fruit., Bourgault R, Oakley AJ, Bewley JD, Wilce MC, Protein Sci. 2005 May;14(5):1233-41. PMID:15840830
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