1rfs: Difference between revisions
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[[Image:1rfs.jpg|left|200px]] | [[Image:1rfs.jpg|left|200px]] | ||
'''RIESKE SOLUBLE FRAGMENT FROM SPINACH''' | {{Structure | ||
|PDB= 1rfs |SIZE=350|CAPTION= <scene name='initialview01'>1rfs</scene>, resolution 1.83Å | |||
|SITE= <scene name='pdbsite=FSB:Fe-S+Binding+Site'>FSB</scene> | |||
|LIGAND= <scene name='pdbligand=FES:FE2/S2 (INORGANIC) CLUSTER'>FES</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''RIESKE SOLUBLE FRAGMENT FROM SPINACH''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1RFS is a [ | 1RFS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RFS OCA]. | ||
==Reference== | ==Reference== | ||
Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein., Carrell CJ, Zhang H, Cramer WA, Smith JL, Structure. 1997 Dec 15;5(12):1613-25. PMID:[http:// | Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein., Carrell CJ, Zhang H, Cramer WA, Smith JL, Structure. 1997 Dec 15;5(12):1613-25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9438861 9438861] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Spinacia oleracea]] | [[Category: Spinacia oleracea]] | ||
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[[Category: iron-sulfur protein]] | [[Category: iron-sulfur protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:51:05 2008'' | ||
Revision as of 14:51, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
RIESKE SOLUBLE FRAGMENT FROM SPINACH
OverviewOverview
BACKGROUND: The cytochrome b6f complex functions in oxygenic photosynthesis as an integral membrane protein complex that mediates coupled electron transfer and proton translocation. The Rieske [2Fe-2S] protein subunit of the complex functions at the electropositive (p) membrane interface as the electron acceptor for plastoquinol and donor for the cytochrome f subunit, and may have a dynamic role in catalyzing electron and proton transfer at the membrane interface. There are significant structure/function similarities to the cytochrome bc1 complex of the respiratory chain. RESULTS: The 1.83 A crystal structure of a 139-residue C-terminal fragment of the Rieske [2Fe-2S] protein, derived from the cytochrome b6f complex of spinach chloroplasts, has been solved by multiwavelength anomalous diffraction. The structure of the fragment comprises two domains: a small 'cluster-binding' subdomain and a large subdomain. The [2Fe-2S] cluster-binding subdomains of the chloroplast and mitochondrial Rieske proteins are virtually identical, whereas the large subdomains are strikingly different despite a common folding topology. A structure-based sequence alignment of the b6f and bc1 groups of Rieske soluble domains is presented. CONCLUSIONS: The segregation of structural conservation and divergence in the cluster-binding and large subdomains of the Rieske protein correlates with the overall relatedness of the cytochrome b6f and bc1 complexes, in which redox domains in the aqueous p phase are dissimilar and those within the membrane are similar. Distinct sequences and surface charge distributions among Rieske large subdomains may provide a signature for interaction with the p-side oxidant protein and for the pH of the intraorganelle compartment.
About this StructureAbout this Structure
1RFS is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.
ReferenceReference
Biological identity and diversity in photosynthesis and respiration: structure of the lumen-side domain of the chloroplast Rieske protein., Carrell CJ, Zhang H, Cramer WA, Smith JL, Structure. 1997 Dec 15;5(12):1613-25. PMID:9438861
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