3vm5: Difference between revisions

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-{{STRUCTURE_3vm5|  PDB=3vm5  |  SCENE=  }}
+==Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris==
-===Recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris===
+<StructureSection load='3vm5' size='340' side='right' caption='[[3vm5]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
-{{ABSTRACT_PUBMED_22613096}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3vm5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryzias_latipes Oryzias latipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VM5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VM5 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vm5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vm5 RCSB], [http://www.ebi.ac.uk/pdbsum/3vm5 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The medaka fish alpha-amylase was expressed and purified. The expression systems were constructed using methylotrophic yeast Pichia pastoris, and the recombinant proteins were secreted into the culture medium. Purified recombinant alpha-amylase exhibited starch hydrolysis activity. The optimal pH, denaturation temperature, and K(M) and V(max) values were determined; chloride ions were essential for enzyme activity. The purified protein was also crystallized and examined by X-ray crystallography. The structure has the (alpha/beta)(8) barrel fold, as do other known alpha-amylases, and the overall structure is very similar to the structure of vertebrate (human and pig) alpha-amylases. A novel expression plasmid was developed. Using this plasmid, high-throughput construction of an expression system by homologous recombination in P. pastoris cells, previously reported for membrane proteins, was successfully applied to the secretory protein.
-==About this Structure==
+Structural and functional characterization of recombinant medaka fish alpha-amylase expressed in yeast Pichia pastoris.,Mizutani K, Toyoda M, Otake Y, Yoshioka S, Takahashi N, Mikami B Biochim Biophys Acta. 2012 May 18. PMID:22613096<ref>PMID:22613096</ref>
-[[3vm5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryzias_latipes Oryzias latipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VM5 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:022613096</ref><references group="xtra"/><references/>
+</div>
+==See Also==
+*[[Amylase|Amylase]]
+*[[User:Gabriel Pons/Sandbox 2|User:Gabriel Pons/Sandbox 2]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Oryzias latipes]]
-[[Category: Mikami, B.]]
+[[Category: Mikami, B]]
-[[Category: Mizutani, K.]]
+[[Category: Mizutani, K]]
-[[Category: Toyoda, M.]]
+[[Category: Toyoda, M]]
 [[Category: Hydrolase]]
 [[Category: Starch hydrolysis]]