1r1c: Difference between revisions

← Older edit Newer edit →

Revision as of 14:45, 20 March 2008

File:1r1c.jpg

, resolution 1.90Å

Ligands:

and

Gene:

AZU OR PA4922 (Pseudomonas aeruginosa)

Coordinates:

save as pdb, mmCIF, xml

PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)

OverviewOverview

Near-UV irradiation of structurally characterized [Re(I)(CO)3(1,10-phenanthroline)(Q107H)](W48F/Y72F/H83Q/Y108W)AzM(II) [Az = Pseudomonas aeruginosa azurin, M = Cu, Zn]/[Co(NH3)5Cl]Cl2 produces a tryptophan radical (W108*) with unprecedented kinetic stability. After rapid formation (k = 2.8 x 106 s-1), the radical persists for more than 5 h at room temperature in the folded ReAzM(II) structure. The absorption spectrum of ReAz(W108*)M(II) exhibits maxima at 512 and 536 nm. Oxidation of K4[Mo(CN)8] by ReAz(W108*)Zn(II) places the W108*/W108 reduction potential in the protein above 0.8 V vs NHE.

About this StructureAbout this Structure

1R1C is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

ReferenceReference

Spectroscopy and reactivity of a photogenerated tryptophan radical in a structurally defined protein environment., Miller JE, Gradinaru C, Crane BR, Di Bilio AJ, Wehbi WA, Un S, Winkler JR, Gray HB, J Am Chem Soc. 2003 Nov 26;125(47):14220-1. PMID:14624538

Page seeded by OCA on Thu Mar 20 13:45:31 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1r1c.jpg|left|200px]]<br /><applet load="1r1c" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1r1c.jpg|left|200px]]
-caption="1r1c, resolution 1.90&Aring;" />
-'''PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)'''<br />
+ {{Structure
+|PDB= 1r1c |SIZE=350|CAPTION= <scene name='initialview01'>1r1c</scene>, resolution 1.90&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CU1:COPPER+(I)+ION'>CU1</scene> and <scene name='pdbligand=REP:(1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I)'>REP</scene>
+|ACTIVITY=
+|GENE= AZU OR PA4922 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
+}}
+'''PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107)'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-R1C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=CU1:'>CU1</scene> and <scene name='pdbligand=REP:'>REP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1C OCA].
+R1C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R1C OCA].
 ==Reference==
-Spectroscopy and reactivity of a photogenerated tryptophan radical in a structurally defined protein environment., Miller JE, Gradinaru C, Crane BR, Di Bilio AJ, Wehbi WA, Un S, Winkler JR, Gray HB, J Am Chem Soc. 2003 Nov 26;125(47):14220-1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14624538 14624538]
+Spectroscopy and reactivity of a photogenerated tryptophan radical in a structurally defined protein environment., Miller JE, Gradinaru C, Crane BR, Di Bilio AJ, Wehbi WA, Un S, Winkler JR, Gray HB, J Am Chem Soc. 2003 Nov 26;125(47):14220-1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14624538 14624538]
 [[Category: Pseudomonas aeruginosa]]
 [[Category: Single protein]]
@@ Line 26: / Line 35: @@
 [[Category: tunneling]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:56 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:45:31 2008''