1qyp: Difference between revisions
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'''THERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURES''' | {{Structure | ||
|PDB= 1qyp |SIZE=350|CAPTION= <scene name='initialview01'>1qyp</scene> | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''THERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURES''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1QYP is a [ | 1QYP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermococcus_celer Thermococcus celer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYP OCA]. | ||
==Reference== | ==Reference== | ||
High-resolution structure of an archaeal zinc ribbon defines a general architectural motif in eukaryotic RNA polymerases., Wang B, Jones DN, Kaine BP, Weiss MA, Structure. 1998 May 15;6(5):555-69. PMID:[http:// | High-resolution structure of an archaeal zinc ribbon defines a general architectural motif in eukaryotic RNA polymerases., Wang B, Jones DN, Kaine BP, Weiss MA, Structure. 1998 May 15;6(5):555-69. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9634694 9634694] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermococcus celer]] | [[Category: Thermococcus celer]] | ||
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[[Category: zn ribbon]] | [[Category: zn ribbon]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:44:32 2008'' | ||
Revision as of 14:44, 20 March 2008
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THERMOCOCCUS CELER RPB9, NMR, 25 STRUCTURES
OverviewOverview
BACKGROUND: Transcriptional initiation and elongation provide control points in gene expression. Eukaryotic RNA polymerase II subunit 9 (RPB9) regulates start-site selection and elongational arrest. RPB9 contains Cys4 Zn(2+)-binding motifs which are conserved in archaea and homologous to those of the general transcription factors TFIIB and TFIIS. RESULTS: The structure of an RPB9 domain from the hyperthermophilic archaeon Thermococcus celer was determined at high resolution by NMR spectroscopy. The structure consists of an apical tetrahedral Zn(2+)-binding site, central beta sheet and disordered loop. Although the structure lacks a globular hydrophobic core, the two surfaces of the beta sheet each contain well ordered aromatic rings engaged in serial edge-to-face interactions. Basic sidechains are clustered near the Zn(2+)-binding site. The disordered loop contains sidechains conserved in TFIIS, including acidic residues essential for the stimulation of transcriptional elongation. CONCLUSIONS: The planar architecture of the RPB9 zinc ribbon-distinct from that of a conventional globular domain-can accommodate significant differences in the alignment of polar, non-polar and charged sidechains. Such divergence is associated with local and non-local changes in structure. The RPB9 structure is distinguished by a fourth beta strand (extending the central beta sheet) in a well ordered N-terminal segment and also differs from TFIIS (but not TFIIB) in the orientation of its apical Zn(2+)-binding site. Cys4 Zn(2+)-binding sites with distinct patterns of polar, non-polar and charged residues are conserved among unrelated RNAP subunits and predicted to form variant zinc ribbons.
About this StructureAbout this Structure
1QYP is a Single protein structure of sequence from Thermococcus celer. Full crystallographic information is available from OCA.
ReferenceReference
High-resolution structure of an archaeal zinc ribbon defines a general architectural motif in eukaryotic RNA polymerases., Wang B, Jones DN, Kaine BP, Weiss MA, Structure. 1998 May 15;6(5):555-69. PMID:9634694
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