1qyc: Difference between revisions
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[[Image:1qyc.gif|left|200px]] | [[Image:1qyc.gif|left|200px]] | ||
'''Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases''' | {{Structure | ||
|PDB= 1qyc |SIZE=350|CAPTION= <scene name='initialview01'>1qyc</scene>, resolution 2.2Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1QYC is a [ | 1QYC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pinus_taeda Pinus taeda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYC OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases and their relationship to isoflavone reductases., Min T, Kasahara H, Bedgar DL, Youn B, Lawrence PK, Gang DR, Halls SC, Park H, Hilsenbeck JL, Davin LB, Lewis NG, Kang C, J Biol Chem. 2003 Dec 12;278(50):50714-23. Epub 2003 Sep 16. PMID:[http:// | Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases and their relationship to isoflavone reductases., Min T, Kasahara H, Bedgar DL, Youn B, Lawrence PK, Gang DR, Halls SC, Park H, Hilsenbeck JL, Davin LB, Lewis NG, Kang C, J Biol Chem. 2003 Dec 12;278(50):50714-23. Epub 2003 Sep 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/13129921 13129921] | ||
[[Category: Pinus taeda]] | [[Category: Pinus taeda]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Youn, B.]] | [[Category: Youn, B.]] | ||
[[Category: ifr]] | [[Category: ifr]] | ||
[[Category: | [[Category: isoflavonoid]] | ||
[[Category: | [[Category: lignan]] | ||
[[Category: nadph-dependent aromatic alcohol | [[Category: nadph-dependent aromatic alcohol reductase]] | ||
[[Category: pcber]] | [[Category: pcber]] | ||
[[Category: plr]] | [[Category: plr]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:44:22 2008'' | ||
Revision as of 14:44, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases
OverviewOverview
Despite the importance of plant lignans and isoflavonoids in human health protection (e.g. for both treatment and prevention of onset of various cancers) as well as in plant biology (e.g. in defense functions and in heartwood development), systematic studies on the enzymes involved in their biosynthesis have only recently begun. In this investigation, three NADPH-dependent aromatic alcohol reductases were comprehensively studied, namely pinoresinol-lariciresinol reductase (PLR), phenylcoumaran benzylic ether reductase (PCBER), and isoflavone reductase (IFR), which are involved in central steps to the various important bioactive lignans and isoflavonoids. Of particular interest was in determining how differing regio- and enantiospecificities are achieved with the different enzymes, despite each apparently going through similar enone intermediates. Initially, the three-dimensional x-ray crystal structures of both PLR_Tp1 and PCBER_Pt1 were solved and refined to 2.5 and 2.2 A resolutions, respectively. Not only do they share high gene sequence similarity, but their structures are similar, having a continuous alpha/beta NADPH-binding domain and a smaller substrate-binding domain. IFR (whose crystal structure is not yet obtained) was also compared (modeled) with PLR and PCBER and was deduced to have the same overall basic structure. The basis for the distinct enantio-specific and regio-specific reactions of PCBER, PLR, and IFR, as well as the reaction mechanism and participating residues involved (as identified by site-directed mutagenesis), are discussed.
About this StructureAbout this Structure
1QYC is a Single protein structure of sequence from Pinus taeda. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases and their relationship to isoflavone reductases., Min T, Kasahara H, Bedgar DL, Youn B, Lawrence PK, Gang DR, Halls SC, Park H, Hilsenbeck JL, Davin LB, Lewis NG, Kang C, J Biol Chem. 2003 Dec 12;278(50):50714-23. Epub 2003 Sep 16. PMID:13129921
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