3o2d: Difference between revisions
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==Crystal structure of HIV-1 primary receptor CD4 in complex with a potent antiviral antibody== | |||
<StructureSection load='3o2d' size='340' side='right' caption='[[3o2d]], [[Resolution|resolution]] 2.19Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3o2d]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O2D FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CD4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o2d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o2d RCSB], [http://www.ebi.ac.uk/pdbsum/3o2d PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ibalizumab is a humanized, anti-CD4 monoclonal antibody. It potently blocks HIV-1 infection and targets an epitope in the second domain of CD4 without interfering with immune functions mediated by interaction of CD4 with major histocompatibility complex (MHC) class II molecules. We report here the crystal structure of ibalizumab Fab fragment in complex with the first two domains (D1-D2) of CD4 at 2.2 A resolution. Ibalizumab grips CD4 primarily by the BC-loop (residues 121-125) of D2, sitting on the opposite side of gp120 and MHC-II binding sites. No major conformational change in CD4 accompanies binding to ibalizumab. Both monovalent and bivalent forms of ibalizumab effectively block viral infection, suggesting that it does not need to crosslink CD4 to exert antiviral activity. While gp120-induced structural rearrangements in CD4 are probably minimal, CD4 structural rigidity is dispensable for ibalizumab inhibition. These results could guide CD4-based immunogen design and lead to a better understanding of HIV-1 entry. | |||
Crystal structure of HIV-1 primary receptor CD4 in complex with a potent antiviral antibody.,Freeman MM, Seaman MS, Rits-Volloch S, Hong X, Kao CY, Ho DD, Chen B Structure. 2010 Dec 8;18(12):1632-41. PMID:21134642<ref>PMID:21134642</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== | ==See Also== | ||
*[[CD4|CD4]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Chen, B | [[Category: Chen, B]] | ||
[[Category: Freeman, M M | [[Category: Freeman, M M]] | ||
[[Category: Ho, D D | [[Category: Ho, D D]] | ||
[[Category: Hong, X | [[Category: Hong, X]] | ||
[[Category: Rits-Volloch, S | [[Category: Rits-Volloch, S]] | ||
[[Category: Seaman, M S | [[Category: Seaman, M S]] | ||
[[Category: Hiv-1 primary receptor]] | [[Category: Hiv-1 primary receptor]] | ||
[[Category: Immune system]] | [[Category: Immune system]] | ||
Revision as of 10:19, 19 December 2014
Crystal structure of HIV-1 primary receptor CD4 in complex with a potent antiviral antibodyCrystal structure of HIV-1 primary receptor CD4 in complex with a potent antiviral antibody
Structural highlights
Publication Abstract from PubMedIbalizumab is a humanized, anti-CD4 monoclonal antibody. It potently blocks HIV-1 infection and targets an epitope in the second domain of CD4 without interfering with immune functions mediated by interaction of CD4 with major histocompatibility complex (MHC) class II molecules. We report here the crystal structure of ibalizumab Fab fragment in complex with the first two domains (D1-D2) of CD4 at 2.2 A resolution. Ibalizumab grips CD4 primarily by the BC-loop (residues 121-125) of D2, sitting on the opposite side of gp120 and MHC-II binding sites. No major conformational change in CD4 accompanies binding to ibalizumab. Both monovalent and bivalent forms of ibalizumab effectively block viral infection, suggesting that it does not need to crosslink CD4 to exert antiviral activity. While gp120-induced structural rearrangements in CD4 are probably minimal, CD4 structural rigidity is dispensable for ibalizumab inhibition. These results could guide CD4-based immunogen design and lead to a better understanding of HIV-1 entry. Crystal structure of HIV-1 primary receptor CD4 in complex with a potent antiviral antibody.,Freeman MM, Seaman MS, Rits-Volloch S, Hong X, Kao CY, Ho DD, Chen B Structure. 2010 Dec 8;18(12):1632-41. PMID:21134642[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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