1qwz: Difference between revisions
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'''Crystal structure of Sortase B from S. aureus complexed with MTSET''' | {{Structure | ||
|PDB= 1qwz |SIZE=350|CAPTION= <scene name='initialview01'>1qwz</scene>, resolution 1.75Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=ETM:2-(TRIMETHYLAMMONIUM)ETHYL THIOL'>ETM</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of Sortase B from S. aureus complexed with MTSET''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1QWZ is a [ | 1QWZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWZ OCA]. | ||
==Reference== | ==Reference== | ||
The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall., Zong Y, Mazmanian SK, Schneewind O, Narayana SV, Structure. 2004 Jan;12(1):105-12. PMID:[http:// | The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall., Zong Y, Mazmanian SK, Schneewind O, Narayana SV, Structure. 2004 Jan;12(1):105-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14725770 14725770] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
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[[Category: transpeptidase]] | [[Category: transpeptidase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:43:51 2008'' | ||
Revision as of 14:43, 20 March 2008
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| , resolution 1.75Å | |||||||
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| Ligands: | , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Sortase B from S. aureus complexed with MTSET
OverviewOverview
Many surface proteins of Gram-positive bacteria, which play important roles during the pathogenesis of human infections, are anchored to the cell wall envelope by a mechanism requiring sortases. Sortase B, a cysteine transpeptidase from Staphylococcus aureus, cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and tethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges. We report here the crystal structures of SrtBDeltaN30 in complex with two active site inhibitors, MTSET and E64, and with the cell wall substrate analog tripleglycine. These structures reveal, for the first time, the active site disposition and the unique Cys-Arg catalytic machinery of the cysteine transpeptidase, and they also provide useful information for the future design of anti-infective agents against sortases.
About this StructureAbout this Structure
1QWZ is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall., Zong Y, Mazmanian SK, Schneewind O, Narayana SV, Structure. 2004 Jan;12(1):105-12. PMID:14725770
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