1qn8: Difference between revisions
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
[[Image:1qn8.gif|left|200px]] | [[Image:1qn8.gif|left|200px]] | ||
'''CRYSTAL STRUCTURE OF THE T(-28) ADENOVIRUS MAJOR LATE PROMOTER TATA BOX VARIANT BOUND TO WILD-TYPE TBP (ARABIDOPSIS THALIANA TBP ISOFORM 2). TATA ELEMENT RECOGNITION BY THE TATA BOX-BINDING PROTEIN HAS BEEN CONSERVED THROUGHOUT EVOLUTION''' | {{Structure | ||
|PDB= 1qn8 |SIZE=350|CAPTION= <scene name='initialview01'>1qn8</scene>, resolution 2.1Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''CRYSTAL STRUCTURE OF THE T(-28) ADENOVIRUS MAJOR LATE PROMOTER TATA BOX VARIANT BOUND TO WILD-TYPE TBP (ARABIDOPSIS THALIANA TBP ISOFORM 2). TATA ELEMENT RECOGNITION BY THE TATA BOX-BINDING PROTEIN HAS BEEN CONSERVED THROUGHOUT EVOLUTION''' | |||
==Overview== | ==Overview== | ||
Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1QN8 is a [ | 1QN8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QN8 OCA]. | ||
==Reference== | ==Reference== | ||
TATA element recognition by the TATA box-binding protein has been conserved throughout evolution., Patikoglou GA, Kim JL, Sun L, Yang SH, Kodadek T, Burley SK, Genes Dev. 1999 Dec 15;13(24):3217-30. PMID:[http:// | TATA element recognition by the TATA box-binding protein has been conserved throughout evolution., Patikoglou GA, Kim JL, Sun L, Yang SH, Kodadek T, Burley SK, Genes Dev. 1999 Dec 15;13(24):3217-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10617571 10617571] | ||
[[Category: Arabidopsis thaliana]] | [[Category: Arabidopsis thaliana]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
Line 22: | Line 31: | ||
[[Category: t (-28) tata box variant]] | [[Category: t (-28) tata box variant]] | ||
[[Category: tata box-binding protein (tbp)]] | [[Category: tata box-binding protein (tbp)]] | ||
[[Category: tbp-tata element | [[Category: tbp-tata element complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:39:59 2008'' |
Revision as of 14:40, 20 March 2008
| |||||||
, resolution 2.1Å | |||||||
---|---|---|---|---|---|---|---|
Coordinates: | save as pdb, mmCIF, xml |
CRYSTAL STRUCTURE OF THE T(-28) ADENOVIRUS MAJOR LATE PROMOTER TATA BOX VARIANT BOUND TO WILD-TYPE TBP (ARABIDOPSIS THALIANA TBP ISOFORM 2). TATA ELEMENT RECOGNITION BY THE TATA BOX-BINDING PROTEIN HAS BEEN CONSERVED THROUGHOUT EVOLUTION
OverviewOverview
Cocrystal structures of wild-type TATA box-binding protein (TBP) recognizing 10 naturally occurring TATA elements have been determined at 2.3-1.8 A resolution, and compared with our 1.9 A resolution structure of TBP bound to the Adenovirus major late promoter (AdMLP) TATA box (5'-TATAAAAG-3'). Minor-groove recognition by the saddle-shaped protein induces the same conformational change in each of these oligonucleotides, despite variations in promoter sequence that reduce the efficiency of transcription initiation. Three molecular mechanisms explain assembly of diverse TBP-TATA element complexes. (1) T --> A and A --> T transversions leave the minor-groove face unchanged, permitting formation of TBP-DNA complexes on many A/T-rich core promoter sequences. (2) Cavities in the interface between TBP and the minor-groove face of the AdMLP TATA box accommodate the exocyclic NH(2) groups of G in a TACA box and in a TATAAG box. (3) Formation of a C:G Hoogsteen basepair in a TATAAAC box eliminates steric clashes that would be produced by the Watson-Crick base pair. We conclude that the structure of the TBP-TATA box complex found at the heart of the polymerase II (pol II) transcription machinery has remained constant over the course of evolution, despite variations in TBP and its DNA targets.
About this StructureAbout this Structure
1QN8 is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
ReferenceReference
TATA element recognition by the TATA box-binding protein has been conserved throughout evolution., Patikoglou GA, Kim JL, Sun L, Yang SH, Kodadek T, Burley SK, Genes Dev. 1999 Dec 15;13(24):3217-30. PMID:10617571
Page seeded by OCA on Thu Mar 20 13:39:59 2008