1qkx: Difference between revisions
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'''ALPHA-SPECTRIN SRC HOMOLOGY 3 DOMAIN, N47A MUTANT IN THE DISTAL LOOP.''' | {{Structure | ||
|PDB= 1qkx |SIZE=350|CAPTION= <scene name='initialview01'>1qkx</scene>, resolution 1.8Å | |||
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'''ALPHA-SPECTRIN SRC HOMOLOGY 3 DOMAIN, N47A MUTANT IN THE DISTAL LOOP.''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1QKX is a [ | 1QKX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QKX OCA]. | ||
==Reference== | ==Reference== | ||
Thermodynamic and structural characterization of Asn and Ala residues in the disallowed II' region of the Ramachandran plot., Vega MC, Martinez JC, Serrano L, Protein Sci. 2000 Dec;9(12):2322-8. PMID:[http:// | Thermodynamic and structural characterization of Asn and Ala residues in the disallowed II' region of the Ramachandran plot., Vega MC, Martinez JC, Serrano L, Protein Sci. 2000 Dec;9(12):2322-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11206053 11206053] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: sh3 domain]] | [[Category: sh3 domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:39:05 2008'' | ||
Revision as of 14:39, 20 March 2008
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ALPHA-SPECTRIN SRC HOMOLOGY 3 DOMAIN, N47A MUTANT IN THE DISTAL LOOP.
OverviewOverview
Residue Asn47 at position L1 of a type II' beta-turn of the alpha-spectrin SH3 domain is located in a disallowed region of the Ramachandran plot (phi = 56 +/- 12, psi = -118 +/- 17). Therefore, it is expected that replacement of Asn47 by Gly should result in a considerable stabilization of the protein. Thermodynamic analysis of the N47G and N47A mutants shows that the change in free energy is small (approximately 0.7 kcal/mol; approximately 3 kJ/mol) and comparable to that found when mutating a Gly to Ala in a alpha-helix or beta-sheet. X-ray structural analysis of these mutants shows that the conformation of the beta-turn does not change upon mutation and, therefore, that there is no relaxation of the structure, nor is there any gain or loss of interactions that could explain the small energy change. Our results indicate that the energetic definition of II' region of the Ramachandran plot (phi = 60 +/- 30, psi = -115 +/- 15) should be revised for at least Ala and Asn in structure validation and protein design.
About this StructureAbout this Structure
1QKX is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
ReferenceReference
Thermodynamic and structural characterization of Asn and Ala residues in the disallowed II' region of the Ramachandran plot., Vega MC, Martinez JC, Serrano L, Protein Sci. 2000 Dec;9(12):2322-8. PMID:11206053
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