1qdc: Difference between revisions

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Revision as of 14:36, 20 March 2008

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MAN(APLHA1-6)MAN(ALPHA1-O)METHYL CONCANAVALIN A COMPLEX

OverviewOverview

The crystal structures of concanavalin A in complex with Man(alpha1-6)Man(alpha1-O)Me and Man(alpha1-3)Man(alpha1-O)Me were determined at resolutions of 2.0 and 2.8 A, respectively. In both structures, the O-1-linked mannose binds in the conserved monosaccharide-binding site. The O-3-linked mannose of Man(alpha1-3)Man(alpha1-O)Me binds in the hydrophobic subsite formed by Tyr-12, Tyr-100, and Leu-99. The shielding of a hydrophobic surface is consistent with the associated large heat capacity change. The O-6-linked mannose of Man(alpha1-6)Man(alpha1-O)Me binds in the same subsite formed by Tyr-12 and Asp-16 as the reducing mannose of the highly specific trimannose Man(alpha1-3)[Man(alpha1-6)]Man(alpha1-O)Me. However, it is much less tightly bound. Its O-2 hydroxyl makes no hydrogen bond with the conserved water 1. Water 1 is present in all the sugar-containing concanavalin A structures and increases the complementarity between the protein-binding surface and the sugar, but is not necessarily a hydrogen-bonding partner. A water analysis of the carbohydrate-binding site revealed a conserved water molecule replacing O-4 on the alpha1-3-linked arm of the trimannose. No such water is found for the reducing or O-6-linked mannose. Our data indicate that the central mannose of Man(alpha1-3)[Man(alpha1-6)]Man(alpha1-O)Me primarily functions as a hinge between the two outer subsites.

About this StructureAbout this Structure

1QDC is a Single protein structure of sequence from Canavalia ensiformis. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structures of Man(alpha1-3)Man(alpha1-O)Me and Man(alpha1-6)Man(alpha1-O)Me in complex with concanavalin A., Bouckaert J, Hamelryck TW, Wyns L, Loris R, J Biol Chem. 1999 Oct 8;274(41):29188-95. PMID:10506175

Page seeded by OCA on Thu Mar 20 13:36:09 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1qdc.jpg|left|200px]]<br /><applet load="1qdc" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1qdc.jpg|left|200px]]
-caption="1qdc, resolution 2.0&Aring;" />
-'''MAN(APLHA1-6)MAN(ALPHA1-O)METHYL CONCANAVALIN A COMPLEX'''<br />
+ {{Structure
+|PDB= 1qdc |SIZE=350|CAPTION= <scene name='initialview01'>1qdc</scene>, resolution 2.0&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''MAN(APLHA1-6)MAN(ALPHA1-O)METHYL CONCANAVALIN A COMPLEX'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-QDC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QDC OCA].
+QDC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QDC OCA].
 ==Reference==
-The crystal structures of Man(alpha1-3)Man(alpha1-O)Me and Man(alpha1-6)Man(alpha1-O)Me in complex with concanavalin A., Bouckaert J, Hamelryck TW, Wyns L, Loris R, J Biol Chem. 1999 Oct 8;274(41):29188-95. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10506175 10506175]
+The crystal structures of Man(alpha1-3)Man(alpha1-O)Me and Man(alpha1-6)Man(alpha1-O)Me in complex with concanavalin A., Bouckaert J, Hamelryck TW, Wyns L, Loris R, J Biol Chem. 1999 Oct 8;274(41):29188-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10506175 10506175]
 [[Category: Canavalia ensiformis]]
 [[Category: Single protein]]
@@ Line 20: / Line 29: @@
 [[Category: MN]]
 [[Category: carbohydrate binding]]
-[[Category: concanavalin a]]
+[[Category: concanavalin some]]
 [[Category: dimannose]]
 [[Category: plant lectin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:38:27 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:36:09 2008''