3fu1: Difference between revisions

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-{{STRUCTURE_3fu1|  PDB=3fu1  |  SCENE=  }}
+==Crystal structure of the major pseudopilin from the type 2 secretion system of Vibrio cholerae==
-===Crystal structure of the major pseudopilin from the type 2 secretion system of Vibrio cholerae===
+<StructureSection load='3fu1' size='340' side='right' caption='[[3fu1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
-{{ABSTRACT_PUBMED_19640838}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3fu1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FU1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3FU1 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">epsG, VC_2730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3fu1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3fu1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3fu1 RCSB], [http://www.ebi.ac.uk/pdbsum/3fu1 PDBsum]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fu/3fu1_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The pseudopilus is a key feature of the type 2 secretion system (T2SS) and is made up of multiple pseudopilins that are similar in fold to the type 4 pilins. However, pilins have disulfide bridges, whereas the major pseudopilins of T2SS do not. A key question is therefore how the pseudopilins, and in particular, the most abundant major pseudopilin, GspG, obtain sufficient stability to perform their function. Crystal structures of Vibrio cholerae, Vibrio vulnificus, and enterohemorrhagic Escherichia coli (EHEC) GspG were elucidated, and all show a calcium ion bound at the same site. Conservation of the calcium ligands fully supports the suggestion that calcium ion binding by the major pseudopilin is essential for the T2SS. Functional studies of GspG with mutated calcium ion-coordinating ligands were performed to investigate this hypothesis and show that in vivo protease secretion by the T2SS is severely impaired. Taking all evidence together, this allows the conclusion that, in complete contrast to the situation in the type 4 pili system homologs, in the T2SS, the major protein component of the central pseudopilus is dependent on calcium ions for activity.
-==Function==
+Calcium is essential for the major pseudopilin in the type 2 secretion system.,Korotkov KV, Gray MD, Kreger A, Turley S, Sandkvist M, Hol WG J Biol Chem. 2009 Sep 18;284(38):25466-70. Epub 2009 Jul 29. PMID:19640838<ref>PMID:19640838</ref>
-[[http://www.uniprot.org/uniprot/GSPG_VIBCH GSPG_VIBCH]] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[3fu1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3FU1 OCA].
+</div>
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:019640838</ref><references group="xtra"/><references/>
+__TOC__
+</StructureSection>
 [[Category: Vibrio cholerae]]
-[[Category: Gray, M D.]]
+[[Category: Gray, M D]]
-[[Category: Hol, W G.J.]]
+[[Category: Hol, W G.J]]
-[[Category: Korotkov, K V.]]
+[[Category: Korotkov, K V]]
-[[Category: Kreger, A.]]
+[[Category: Kreger, A]]
-[[Category: Sandkvist, M.]]
+[[Category: Sandkvist, M]]
-[[Category: Turley, S.]]
+[[Category: Turley, S]]
 [[Category: Complex]]
 [[Category: General secretory pathway]]