3ahu: Difference between revisions

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-{{STRUCTURE_3ahu|  PDB=3ahu  |  SCENE=  }}
+==Crystal structure of YmaH (Hfq) from Bacillus subtilis in complex with an RNA aptamer.==
-===Crystal structure of YmaH (Hfq) from Bacillus subtilis in complex with an RNA aptamer.===
+<StructureSection load='3ahu' size='340' side='right' caption='[[3ahu]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-{{ABSTRACT_PUBMED_20445260}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3ahu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AHU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3AHU FirstGlance]. <br>
+</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSU17340, hfq, ymaH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ahu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ahu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ahu RCSB], [http://www.ebi.ac.uk/pdbsum/3ahu PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The Hfq protein is a hexameric RNA-binding protein which regulates gene expression by binding to RNA under the influence of diverse environmental stresses. Its ring structure binds various types of RNA, including mRNA and sRNA. RNA-bound structures of Hfq from Escherichia coli and Staphylococcus aureus have been revealed to have poly(A) RNA at the distal site and U-rich RNA at the proximal site, respectively. Here, crystals of a complex of the Bacillus subtilis Hfq protein with an A/G-repeat 7-mer RNA (Hfq-RNA) that were prepared using the hanging-drop vapour-diffusion technique are reported. The type 1 Hfq-RNA crystals belonged to space group I422, with unit-cell parameters a = b = 123.70, c = 119.13 A, while the type 2 Hfq-RNA crystals belonged to space group F222, with unit-cell parameters a = 91.92, b = 92.50, c = 114.92 A. Diffraction data were collected to a resolution of 2.20 A from both crystal forms. The hexameric structure of the Hfq protein was clearly shown by self-rotation analysis.
-==About this Structure==
+Expression, crystallization and preliminary crystallographic analysis of RNA-binding protein Hfq (YmaH) from Bacillus subtilis in complex with an RNA aptamer.,Baba S, Someya T, Kawai G, Nakamura K, Kumasaka T Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 May 1;66(Pt, 5):563-6. Epub 2010 Apr 30. PMID:20445260<ref>PMID:20445260</ref>
-[[3ahu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AHU OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:020445260</ref><references group="xtra"/><references/>
+</div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Bacillus subtilis]]
-[[Category: Baba, S.]]
+[[Category: Baba, S]]
-[[Category: Kawai, G.]]
+[[Category: Kawai, G]]
-[[Category: Kumasaka, T.]]
+[[Category: Kumasaka, T]]
-[[Category: Nakamura, K.]]
+[[Category: Nakamura, K]]
-[[Category: Someya, T.]]
+[[Category: Someya, T]]
 [[Category: Protein-rna complex]]
 [[Category: Sm-like motif]]
 [[Category: Translation-rna complex]]