1q10: Difference between revisions
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[[Image:1q10.gif|left|200px]] | [[Image:1q10.gif|left|200px]] | ||
'''Ensemble of 40 Structures of the Dimeric Mutant of the B1 Domain of Streptococcal Protein G''' | {{Structure | ||
|PDB= 1q10 |SIZE=350|CAPTION= <scene name='initialview01'>1q10</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= SPG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Streptococcus sp. group G]) | |||
}} | |||
'''Ensemble of 40 Structures of the Dimeric Mutant of the B1 Domain of Streptococcal Protein G''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1Q10 is a [ | 1Q10 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptococcus_sp._group_g Streptococcus sp. group g]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q10 OCA]. | ||
==Reference== | ==Reference== | ||
A protein contortionist: core mutations of GB1 that induce dimerization and domain swapping., Byeon IJ, Louis JM, Gronenborn AM, J Mol Biol. 2003 Oct 10;333(1):141-52. PMID:[http:// | A protein contortionist: core mutations of GB1 that induce dimerization and domain swapping., Byeon IJ, Louis JM, Gronenborn AM, J Mol Biol. 2003 Oct 10;333(1):141-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14516749 14516749] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptococcus sp. group g]] | [[Category: Streptococcus sp. group g]] | ||
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[[Category: Gronenborn, A M.]] | [[Category: Gronenborn, A M.]] | ||
[[Category: Louis, J M.]] | [[Category: Louis, J M.]] | ||
[[Category: core | [[Category: core mutant]] | ||
[[Category: domain-swapping]] | [[Category: domain-swapping]] | ||
[[Category: gb1]] | [[Category: gb1]] | ||
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[[Category: oligomerization]] | [[Category: oligomerization]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:31:35 2008'' | ||
Revision as of 14:31, 20 March 2008
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| Gene: | SPG (Streptococcus sp. group G) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Ensemble of 40 Structures of the Dimeric Mutant of the B1 Domain of Streptococcal Protein G
OverviewOverview
Immunoglobulin-binding domain B1 of streptococcal protein G (GB1), a small (56 residues), stable, single-domain protein, is one of the most extensively used model systems in the area of protein folding and design. Recently, NMR and X-ray structures of a quintuple GB1 core mutant (L5V/A26F/F30V/Y33F/A34F) that showed an unexpected, intertwined tetrameric architecture were determined. Here, we report the NMR structure of another mutant, derived from the tetramer by reverting the single amino acid position F26 back to the wild-type sequence A26. The structure reveals a domain-swapped dimer that involves exchange of the second beta-hairpin. The resulting overall structure comprises an eight-stranded beta-sheet whose concave side is covered by two alpha helices. The dimer dissociates into a partially folded, monomeric species with a dissociation constant of 93(+/-10)microM.
About this StructureAbout this Structure
1Q10 is a Single protein structure of sequence from Streptococcus sp. group g. Full crystallographic information is available from OCA.
ReferenceReference
A protein contortionist: core mutations of GB1 that induce dimerization and domain swapping., Byeon IJ, Louis JM, Gronenborn AM, J Mol Biol. 2003 Oct 10;333(1):141-52. PMID:14516749
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