1q07: Difference between revisions

← Older edit Newer edit →

Revision as of 14:31, 20 March 2008

File:1q07.gif

, resolution 2.50Å

Ligands:

Gene:

CUER (Escherichia coli)

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator

OverviewOverview

The earliest of a series of copper efflux genes in Escherichia coli are controlled by CueR, a member of the MerR family of transcriptional activators. Thermodynamic calibration of CueR reveals a zeptomolar (10(-21) molar) sensitivity to free Cu+, which is far less than one atom per cell. Atomic details of this extraordinary sensitivity and selectivity for +1transition-metal ions are revealed by comparing the crystal structures of CueR and a Zn2+-sensing homolog, ZntR. An unusual buried metal-receptor site in CueR restricts the metal to a linear, two-coordinate geometry and uses helix-dipole and hydrogen-bonding interactions to enhance metal binding. This binding mode is rare among metalloproteins but well suited for an ultrasensitive genetic switch.

About this StructureAbout this Structure

1Q07 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR., Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragon A, Science. 2003 Sep 5;301(5638):1383-7. PMID:12958362

Page seeded by OCA on Thu Mar 20 13:31:18 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1q07.gif|left|200px]]<br /><applet load="1q07" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1q07.gif|left|200px]]
-caption="1q07, resolution 2.50&Aring;" />
-'''Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator'''<br />
+ {{Structure
+|PDB= 1q07 |SIZE=350|CAPTION= <scene name='initialview01'>1q07</scene>, resolution 2.50&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=AU:GOLD ION'>AU</scene>
+|ACTIVITY=
+|GENE= CUER ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+}}
+'''Crystal structure of the Au(I) form of E. coli CueR, a copper efflux regulator'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-Q07 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=AU:'>AU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q07 OCA].
+Q07 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q07 OCA].
 ==Reference==
-Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR., Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragon A, Science. 2003 Sep 5;301(5638):1383-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12958362 12958362]
+Molecular basis of metal-ion selectivity and zeptomolar sensitivity by CueR., Changela A, Chen K, Xue Y, Holschen J, Outten CE, O'Halloran TV, Mondragon A, Science. 2003 Sep 5;301(5638):1383-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12958362 12958362]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: merr family transcriptional regulator]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:31:18 2008''