2xxl: Difference between revisions

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-{{STRUCTURE_2xxl|  PDB=2xxl  |  SCENE=  }}
+==CRYSTAL STRUCTURE OF DROSOPHILA GRASS CLIP SERINE PROTEASE OF TOLL PATHWAY==
-===CRYSTAL STRUCTURE OF DROSOPHILA GRASS CLIP SERINE PROTEASE OF TOLL PATHWAY===
+<StructureSection load='2xxl' size='340' side='right' caption='[[2xxl]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-{{ABSTRACT_PUBMED_21310954}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2xxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XXL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2XXL FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2xxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xxl OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2xxl RCSB], [http://www.ebi.ac.uk/pdbsum/2xxl PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Grass is a clip domain serine protease (SP) involved in a proteolytic cascade triggering the Toll pathway activation of Drosophila during an immune response. Epistasic studies position it downstream of the apical protease ModSP and upstream of the terminal protease Spaetzle-processing enzyme. Here, we report the crystal structure of Grass zymogen. We found that Grass displays a rather deep active site cleft comparable with that of proteases of coagulation and complement cascades. A key distinctive feature is the presence of an additional loop (75-loop) in the proximity of the activation site localized on a protruding loop. All biochemical attempts to hydrolyze the activation site of Grass failed, strongly suggesting restricted access to this region. The 75-loop is thus proposed to constitute an original mechanism to prevent spontaneous activation. A comparison of Grass with clip serine proteases of known function involved in analogous proteolytic cascades allowed us to define two groups, according to the presence of the 75-loop and the conformation of the clip domain. One group (devoid of the 75-loop) contains penultimate proteases whereas the other contains terminal proteases. Using this classification, Grass appears to be a terminal protease. This result is evaluated according to the genetic data documenting Grass function.
-==About this Structure==
+Structure-function analysis of grass clip serine protease involved in Drosophila Toll pathway activation.,Kellenberger C, Leone P, Coquet L, Jouenne T, Reichhart JM, Roussel A J Biol Chem. 2011 Apr 8;286(14):12300-7. Epub 2011 Feb 10. PMID:21310954<ref>PMID:21310954</ref>
-[[2xxl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XXL OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:021310954</ref><references group="xtra"/><references/>
+</div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Drosophila melanogaster]]
 [[Category: Trypsin]]
-[[Category: Coquet, L.]]
+[[Category: Coquet, L]]
-[[Category: Jouenne, T.]]
+[[Category: Jouenne, T]]
-[[Category: Kellenberger, C.]]
+[[Category: Kellenberger, C]]
-[[Category: Leone, P.]]
+[[Category: Leone, P]]
-[[Category: Reichhart, J M.]]
+[[Category: Reichhart, J M]]
-[[Category: Roussel, A.]]
+[[Category: Roussel, A]]
 [[Category: Hydrolase]]
 [[Category: Innate immunity]]