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{{STRUCTURE_2d55|  PDB=2d55  |  SCENE=  }}
==Structural, physical and biological characteristics of RNA.DNA binding agent N8-actinomycin D==
===Structural, physical and biological characteristics of RNA.DNA binding agent N8-actinomycin D===
<StructureSection load='2d55' size='340' side='right' caption='[[2d55]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
{{ABSTRACT_PUBMED_1593629}}
== Structural highlights ==
<table><tr><td colspan='2'>[[2d55]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1d55 1d55]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D55 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2D55 FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DVA:D-VALINE'>DVA</scene>, <scene name='pdbligand=MVA:N-METHYLVALINE'>MVA</scene>, <scene name='pdbligand=PXZ:2-AMINO-1,9-DICARBONYL-4,6-DIMETHYL-10-DEHYDRO-PHENOXAZIN-3-ONE'>PXZ</scene>, <scene name='pdbligand=SAR:SARCOSINE'>SAR</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1a7z|1a7z]], [[209d|209d]], [[1unm|1unm]], [[1i3w|1i3w]], [[1a7y|1a7y]], [[1fja|1fja]], [[173d|173d]], [[1dsc|1dsc]], [[1l1v|1l1v]], [[316d|316d]], [[1dsd|1dsd]], [[1mnv|1mnv]], [[1unj|1unj]], [[1ovf|1ovf]], [[1qfi|1qfi]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2d55 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d55 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2d55 RCSB], [http://www.ebi.ac.uk/pdbsum/2d55 PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structures of the 2:1 complex of the self-complementary DNA octamer d(GAAGCTTC) with actinomycin D has been determined at 3.0 A resolution. This is the first example of a crystal structure of a DNA-drug complex in which the drug intercalates into the middle of a relatively long DNA segment. The results finally confirmed the DNA-actinomycin intercalation model proposed by Sobell &amp; co-workers in 1971. The DNA molecule adopts a severely distorted and slightly kinked B-DNA-like structure with an actinomycin D molecule intercalated in the middle sequence, GC. The two cyclic depsipeptides, which differ from each other in overall conformation, lie in the minor groove. The complex is further stabilized by forming base-peptide and chromophore-backbone hydrogen bonds. The DNA helix appears to be unwound by rotating one of the base-pairs at the intercalation site. This single base-pair unwinding motion generates a unique asymmetrically wound helix at the binding site of the drug, i.e. the helix is loosened at one end of the intercalation site and tightened at the other end. The large unwinding of the DNA by the drug intercalation is absorbed mostly in a few residues adjacent to the intercalation site. The asymmetrical twist of the DNA helix, the overall conformation of the two cyclic depsipeptides and their interaction mode with DNA are correlated to each other and rationally explained.


==About this Structure==
Crystal structure of the 2:1 complex between d(GAAGCTTC) and the anticancer drug actinomycin D.,Kamitori S, Takusagawa F J Mol Biol. 1992 May 20;225(2):445-56. PMID:1593629<ref>PMID:1593629</ref>
[[2d55]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_antibioticus Streptomyces antibioticus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1d55 1d55]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D55 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:001593629</ref><references group="xtra"/><references/>
</div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Streptomyces antibioticus]]
[[Category: Streptomyces antibioticus]]
[[Category: Carlson, R G.]]
[[Category: Carlson, R G]]
[[Category: Chu, W.]]
[[Category: Chu, W]]
[[Category: Shinomiya, M.]]
[[Category: Shinomiya, M]]
[[Category: Takusagawa, F.]]
[[Category: Takusagawa, F]]
[[Category: Weaver, R F.]]
[[Category: Weaver, R F]]
[[Category: Actinomycin d]]
[[Category: Actinomycin d]]
[[Category: Anti cancer]]
[[Category: Anti cancer]]

Revision as of 15:54, 18 December 2014

Structural, physical and biological characteristics of RNA.DNA binding agent N8-actinomycin DStructural, physical and biological characteristics of RNA.DNA binding agent N8-actinomycin D

Structural highlights

2d55 is a 3 chain structure with sequence from Streptomyces antibioticus. This structure supersedes the now removed PDB entry 1d55. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:, , ,
Resources:FirstGlance, OCA, RCSB, PDBsum

Publication Abstract from PubMed

The crystal structures of the 2:1 complex of the self-complementary DNA octamer d(GAAGCTTC) with actinomycin D has been determined at 3.0 A resolution. This is the first example of a crystal structure of a DNA-drug complex in which the drug intercalates into the middle of a relatively long DNA segment. The results finally confirmed the DNA-actinomycin intercalation model proposed by Sobell & co-workers in 1971. The DNA molecule adopts a severely distorted and slightly kinked B-DNA-like structure with an actinomycin D molecule intercalated in the middle sequence, GC. The two cyclic depsipeptides, which differ from each other in overall conformation, lie in the minor groove. The complex is further stabilized by forming base-peptide and chromophore-backbone hydrogen bonds. The DNA helix appears to be unwound by rotating one of the base-pairs at the intercalation site. This single base-pair unwinding motion generates a unique asymmetrically wound helix at the binding site of the drug, i.e. the helix is loosened at one end of the intercalation site and tightened at the other end. The large unwinding of the DNA by the drug intercalation is absorbed mostly in a few residues adjacent to the intercalation site. The asymmetrical twist of the DNA helix, the overall conformation of the two cyclic depsipeptides and their interaction mode with DNA are correlated to each other and rationally explained.

Crystal structure of the 2:1 complex between d(GAAGCTTC) and the anticancer drug actinomycin D.,Kamitori S, Takusagawa F J Mol Biol. 1992 May 20;225(2):445-56. PMID:1593629[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. ↑ Kamitori S, Takusagawa F. Crystal structure of the 2:1 complex between d(GAAGCTTC) and the anticancer drug actinomycin D. J Mol Biol. 1992 May 20;225(2):445-56. PMID:1593629

2d55, resolution 3.00Å

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