1prl: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1prl.gif|left|200px]] | [[Image:1prl.gif|left|200px]] | ||
'''TWO BINDING ORIENTATIONS FOR PEPTIDES TO SRC SH3 DOMAIN: DEVELOPMENT OF A GENERAL MODEL FOR SH3-LIGAND INTERACTIONS''' | {{Structure | ||
|PDB= 1prl |SIZE=350|CAPTION= <scene name='initialview01'>1prl</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= SYNTHETIC OLIGO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]) | |||
}} | |||
'''TWO BINDING ORIENTATIONS FOR PEPTIDES TO SRC SH3 DOMAIN: DEVELOPMENT OF A GENERAL MODEL FOR SH3-LIGAND INTERACTIONS''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1PRL is a [ | 1PRL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRL OCA]. | ||
==Reference== | ==Reference== | ||
Two binding orientations for peptides to the Src SH3 domain: development of a general model for SH3-ligand interactions., Feng S, Chen JK, Yu H, Simon JA, Schreiber SL, Science. 1994 Nov 18;266(5188):1241-7. PMID:[http:// | Two binding orientations for peptides to the Src SH3 domain: development of a general model for SH3-ligand interactions., Feng S, Chen JK, Yu H, Simon JA, Schreiber SL, Science. 1994 Nov 18;266(5188):1241-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7526465 7526465] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 20: | Line 29: | ||
[[Category: complex (signal transduction/peptide)]] | [[Category: complex (signal transduction/peptide)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:28:02 2008'' | ||
Revision as of 14:28, 20 March 2008
| |||||||
| Gene: | SYNTHETIC OLIGO (Gallus gallus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TWO BINDING ORIENTATIONS FOR PEPTIDES TO SRC SH3 DOMAIN: DEVELOPMENT OF A GENERAL MODEL FOR SH3-LIGAND INTERACTIONS
OverviewOverview
Solution structures of two Src homology 3 (SH3) domain-ligand complexes have been determined by nuclear magnetic resonance. Each complex consists of the SH3 domain and a nine-residue proline-rich peptide selected from a large library of ligands prepared by combinatorial synthesis. The bound ligands adopt a left-handed polyproline type II (PPII) helix, although the amino to carboxyl directionalities of their helices are opposite. The peptide orientation is determined by a salt bridge formed by the terminal arginine residues of the ligands and the conserved aspartate-99 of the SH3 domain. Residues at positions 3, 4, 6, and 7 of both peptides also intercalate into the ligand-binding site; however, the respective proline and nonproline residues show exchanged binding positions in the two complexes. These structural results led to a model for the interactions of SH3 domains with proline-rich peptides that can be used to predict critical residues in complexes of unknown structure. The model was used to identify correctly both the binding orientation and the contact and noncontact residues of a peptide derived from the nucleotide exchange factor Sos in association with the amino-terminal SH3 domain of the adaptor protein Grb2.
About this StructureAbout this Structure
1PRL is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
ReferenceReference
Two binding orientations for peptides to the Src SH3 domain: development of a general model for SH3-ligand interactions., Feng S, Chen JK, Yu H, Simon JA, Schreiber SL, Science. 1994 Nov 18;266(5188):1241-7. PMID:7526465
Page seeded by OCA on Thu Mar 20 13:28:02 2008