3m5g: Difference between revisions

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-{{STRUCTURE_3m5g|  PDB=3m5g  |  SCENE=  }}
+==Crystal structure of a H7 influenza virus hemagglutinin==
-===Crystal structure of a H7 influenza virus hemagglutinin===
+<StructureSection load='3m5g' size='340' side='right' caption='[[3m5g]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-{{ABSTRACT_PUBMED_20824086}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3m5g]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M5G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3M5G FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3m5h|3m5h]], [[3m5i|3m5i]], [[3m5j|3m5j]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11320 Influenza A virus])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3m5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3m5g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3m5g RCSB], [http://www.ebi.ac.uk/pdbsum/3m5g PDBsum]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/m5/3m5g_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Human infections with subtype H7 avian influenza viruses have been reported as early as 1979. In 1996, a genetically stable 24-nucleotide deletion emerged in North American H7 influenza virus hemagglutinins, resulting in an eight amino acid deletion in the receptor-binding site. The continuous circulation of these viruses in live bird markets, as well as its documented ability to infect humans, raises the question of how these viruses achieve structural stability and functionality. Here we report a detailed molecular analysis of the receptor binding site of the North American lineage subtype H7N2 virus A/New York/107/2003 (NY107), including complexes with an avian receptor analog (3'-sialyl-N-acetyllactosamine, 3'SLN) and two human receptor analogs (6'-sialyl-N-acetyllactosamine, 6'SLN; sialyllacto-N-tetraose b, LSTb). Structural results suggest a novel mechanism by which residues Arg220 and Arg229 (H3 numbering) are used to compensate for the deletion of the 220-loop and form interactions with the receptor analogs. Glycan microarray results reveal that NY107 maintains an avian-type (alpha2-3) receptor binding profile, with only moderate binding to human-type (alpha2-6) receptor. Thus despite its dramatically altered receptor binding site, this HA maintains functionality and confirms a need for continued influenza virus surveillance of avian and other animal reservoirs to define their zoonotic potential.
-==About this Structure==
+Structures of receptor complexes of a North American H7N2 influenza hemagglutinin with a loop deletion in the receptor binding site.,Yang H, Chen LM, Carney PJ, Donis RO, Stevens J PLoS Pathog. 2010 Sep 2;6(9):e1001081. PMID:20824086<ref>PMID:20824086</ref>
-[[3m5g]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_a_virus Influenza a virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3M5G OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
 ==See Also==
 *[[Hemagglutinin|Hemagglutinin]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:020824086</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Influenza a virus]]
-[[Category: Carney, P J.]]
+[[Category: Carney, P J]]
-[[Category: Chen, L M.]]
+[[Category: Chen, L M]]
-[[Category: Donis, R O.]]
+[[Category: Donis, R O]]
-[[Category: Stevens, J.]]
+[[Category: Stevens, J]]
-[[Category: Yang, H.]]
+[[Category: Yang, H]]
 [[Category: Envelope protein]]
 [[Category: Fusion protein]]