1p9d: Difference between revisions

Revision as of 14:21, 20 March 2008

Gene:

PSMD4 OR MCB1 (Homo sapiens), RAD23A (Homo sapiens)

Coordinates:

High-resolution structure of the complex of HHR23A ubiquitin-like domain and the C-terminal ubiquitin-interacting motif of proteasome subunit S5a

OverviewOverview

HHR23A, a protein implicated in nucleotide excision repair, belongs to a class of proteins containing both a ubiquitin-like (Ubl) domain and one or more ubiquitin-associated (UBA) domains, suggesting a role in the ubiquitin-proteasome pathway as well. The Ubl domain binds with high affinity to the second ubiquitin-interacting motif (UIM) of the S5a subunit of the proteasome. Here we present the solution structures of the HHR23A Ubl domain, the second UIM of S5a (UIM-2), and the Ubl:S5a-UIM-2 complex. The HHR23A Ubl domain is structurally similar to ubiquitin. The S5a UIM forms an alpha-helix with an unexpected hairpin loop that contributes to the binding interface with Ubl. The molecular determinants of the Ubl-proteasome interaction are revealed by analysis of the structures, chemical shift mapping, mutant binding studies and sequence conservation.

About this StructureAbout this Structure

1P9D is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural determinants for the binding of ubiquitin-like domains to the proteasome., Mueller TD, Feigon J, EMBO J. 2003 Sep 15;22(18):4634-45. PMID:12970176

Page seeded by OCA on Thu Mar 20 13:21:36 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1p9d.gif|left|200px]]<br /><applet load="1p9d" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1p9d.gif|left|200px]]
-caption="1p9d" />
-'''High-resolution structure of the complex of HHR23A ubiquitin-like domain and the C-terminal ubiquitin-interacting motif of proteasome subunit S5a'''<br />
+ {{Structure
+|PDB= 1p9d |SIZE=350|CAPTION= <scene name='initialview01'>1p9d</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE= PSMD4 OR MCB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RAD23A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+}}
+'''High-resolution structure of the complex of HHR23A ubiquitin-like domain and the C-terminal ubiquitin-interacting motif of proteasome subunit S5a'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-P9D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9D OCA].
+P9D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9D OCA].
 ==Reference==
-Structural determinants for the binding of ubiquitin-like domains to the proteasome., Mueller TD, Feigon J, EMBO J. 2003 Sep 15;22(18):4634-45. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12970176 12970176]
+Structural determinants for the binding of ubiquitin-like domains to the proteasome., Mueller TD, Feigon J, EMBO J. 2003 Sep 15;22(18):4634-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12970176 12970176]
 [[Category: Homo sapiens]]
 [[Category: Protein complex]]
@@ Line 17: / Line 26: @@
 [[Category: protein-peptide complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:30 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:21:36 2008''