3i2j: Difference between revisions

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-{{STRUCTURE_3i2j|  PDB=3i2j  |  SCENE=  }}
+==Cocaine Esterase, wild type, without a ligand==
-===Cocaine Esterase, wild type, without a ligand===
+<StructureSection load='3i2j' size='340' side='right' caption='[[3i2j]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
-{{ABSTRACT_PUBMED_20436035}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3i2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_sp._mb1_'bresler_1999' Rhodococcus sp. mb1 'bresler 1999']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3I2J FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ju3|1ju3]], [[1ju4|1ju4]], [[1l7r|1l7r]], [[1l7q|1l7q]], [[3i2f|3i2f]], [[3i2g|3i2g]], [[3i2h|3i2h]], [[3i2i|3i2i]], [[3i2k|3i2k]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cocE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=104109 Rhodococcus sp. MB1 'Bresler 1999'])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3i2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3i2j RCSB], [http://www.ebi.ac.uk/pdbsum/3i2j PDBsum]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i2/3i2j_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Cocaine is considered to be the most addictive of all substances of abuse and mediates its effects by inhibiting monoamine transporters, primarily the dopamine transporters. There are currently no small molecules that can be used to combat its toxic and addictive properties, in part because of the difficulty of developing compounds that inhibit cocaine binding without having intrinsic effects on dopamine transport. Most of the effective cocaine inhibitors also display addictive properties. We have recently reported the use of cocaine esterase (CocE) to accelerate the removal of systemic cocaine and to prevent cocaine-induced lethality. However, wild-type CocE is relatively unstable at physiological temperatures (tau(1/2) approximately 13 min at 37 degrees C), presenting challenges for its development as a viable therapeutic agent. We applied computational approaches to predict mutations to stabilize CocE and showed that several of these have increased stability both in vitro and in vivo, with the most efficacious mutant (T172R/G173Q) extending half-life up to 370 min. Here we present novel X-ray crystallographic data on these mutants that provide a plausible model for the observed enhanced stability. We also more extensively characterize the previously reported variants and report on a new stabilizing mutant, L169K. The improved stability of these engineered CocE enzymes will have a profound influence on the use of this protein to combat cocaine-induced toxicity and addiction in humans.
-==About this Structure==
+Structural analysis of thermostabilizing mutations of cocaine esterase.,Narasimhan D, Nance MR, Gao D, Ko MC, Macdonald J, Tamburi P, Yoon D, Landry DM, Woods JH, Zhan CG, Tesmer JJ, Sunahara RK Protein Eng Des Sel. 2010 Jul;23(7):537-47. Epub 2010 Apr 30. PMID:20436035<ref>PMID:20436035</ref>
-[[3i2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodococcus_sp._mb1_'bresler_1999' Rhodococcus sp. mb1 'bresler 1999']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I2J OCA].
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
 ==See Also==
 *[[Cocaine esterase|Cocaine esterase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:020436035</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Rhodococcus sp. mb1 'bresler 1999']]
-[[Category: Nance, M R.]]
+[[Category: Nance, M R]]
-[[Category: Tesmer, J J.G.]]
+[[Category: Tesmer, J J.G]]
 [[Category: Alpha/beta hydrolase]]
 [[Category: Hydrolase]]