1p6t: Difference between revisions

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Revision as of 14:20, 20 March 2008

File:1p6t.gif

Gene:

YVGX (Bacillus subtilis)

Activity:

Copper-exporting ATPase, with EC number 3.6.3.4

Coordinates:

save as pdb, mmCIF, xml

Structure characterization of the water soluble region of P-type ATPase CopA from Bacillus subtilis

OverviewOverview

The solution structure of the N-terminal region (151 amino acids) of a copper ATPase, CopA, from Bacillus subtilis, is reported here. It consists of two domains, CopAa and CopAb, linked by two amino acids. It is found that the two domains, which had already been separately characterized, interact one to the other through a hydrogen bond network and a few hydrophobic interactions, forming a single rigid body. The two metal binding sites are far from one another, and the short link between the domains prevents them from interacting. This and the surface electrostatic potential suggest that each domain receives copper from the copper chaperone, CopZ, independently and transfers it to the membrane binding site of CopA. The affinity constants of silver(I) and copper(I) are similar for the two sites as monitored by NMR. Because the present construct "domain-short link-domain" is shared also by the last two domains of the eukaryotic copper ATPases and several residues at the interface between the two domains are conserved, the conclusions of the present study have general validity for the understanding of the function of copper ATPases.

About this StructureAbout this Structure

1P6T is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis., Banci L, Bertini I, Ciofi-Baffoni S, Gonnelli L, Su XC, J Biol Chem. 2003 Dec 12;278(50):50506-13. Epub 2003 Sep 27. PMID:14514665

Page seeded by OCA on Thu Mar 20 13:20:38 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1p6t.gif|left|200px]]<br /><applet load="1p6t" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1p6t.gif|left|200px]]
-caption="1p6t" />
-'''Structure characterization of the water soluble region of P-type ATPase CopA from Bacillus subtilis'''<br />
+ {{Structure
+|PDB= 1p6t |SIZE=350|CAPTION= <scene name='initialview01'>1p6t</scene>
+|SITE=
+|LIGAND=
+|ACTIVITY= [http://en.wikipedia.org/wiki/Copper-exporting_ATPase Copper-exporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.4 3.6.3.4]
+|GENE= YVGX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
+}}
+'''Structure characterization of the water soluble region of P-type ATPase CopA from Bacillus subtilis'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-P6T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Active as [http://en.wikipedia.org/wiki/Copper-exporting_ATPase Copper-exporting ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.4 3.6.3.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6T OCA].
+P6T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6T OCA].
 ==Reference==
-Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis., Banci L, Bertini I, Ciofi-Baffoni S, Gonnelli L, Su XC, J Biol Chem. 2003 Dec 12;278(50):50506-13. Epub 2003 Sep 27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14514665 14514665]
+Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis., Banci L, Bertini I, Ciofi-Baffoni S, Gonnelli L, Su XC, J Biol Chem. 2003 Dec 12;278(50):50506-13. Epub 2003 Sep 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14514665 14514665]
 [[Category: Bacillus subtilis]]
 [[Category: Copper-exporting ATPase]]
@@ Line 22: / Line 31: @@
 [[Category: copa; p-type atpase; water-soluble region; beta-alpha-beta-beta-alpha-beta fold; nmr]]
 [[Category: spine]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
 [[Category: structural proteomics in europe]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:42 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:20:38 2008''