104d: Difference between revisions
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==DNA DUPLEXES FLANKED BY HYBRID DUPLEXES: THE SOLUTION STRUCTURE OF CHIMERIC JUNCTIONS IN== | |||
<StructureSection load='104d' size='340' side='right' caption='[[104d]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[104d]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=104D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=104D FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=104d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=104d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=104d RCSB], [http://www.ebi.ac.uk/pdbsum/104d PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Hybrid duplexes and chimeric duplexes containing hybrid segments linked to pure DNA (or pure RNA) segments are involved in transcription and replication, as well as reverse transcription. A complete understanding of the mechanism of these processes requires detailed information on such duplexes and the junctions between duplexes of differing structure. Using two-dimensional NMR, restrained molecular dynamics and mechanics, and back-calculation refinement against the nuclear Overhauser effect spectra at various mixing times, we have determined the solution structure of the chimeric duplex [r(cgcg)d-(TATACGCG)]2 containing a pure DNA segment in the center of a hybrid duplex. The solution structure differs from the previously determined X-ray structure of the analogous duplex [r(gcg)d(TATACGC)]2, which was found to be A-form throughout [Wang, A.H.-J., et al. (1982) Nature 299, 601-604]. The basic features of the solution structure are (a) the RNA residues are all A-form with C3'-endo sugar conformations, (b) the central DNA segment is B-form, (c) the transition from A-form RNA sugar conformations to B-form DNA sugar conformations involves only the dT5 base step, and (d) although the sugar conformations of the DNA residues A6-G12 are closer to B-form, the basic helical properties of the peripheral RNA.DNA hybrid segments are closer to typical A-form than to B-form. | |||
DNA duplexes flanked by hybrid duplexes: the solution structure of chimeric junctions in [r(cgcg)d(TATACGCG)]2.,Zhu L, Salazar M, Reid BR Biochemistry. 1995 Feb 21;34(7):2372-80. PMID:7857947<ref>PMID:7857947</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Protein Data Bank|Protein Data Bank]] | *[[Protein Data Bank|Protein Data Bank]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
[[Category: Reid, B R | </StructureSection> | ||
[[Category: Salazar, M | [[Category: Reid, B R]] | ||
[[Category: Zhu, L | [[Category: Salazar, M]] | ||
[[Category: Zhu, L]] | |||
[[Category: Dna]] | [[Category: Dna]] | ||
[[Category: Dna-rna complex]] | [[Category: Dna-rna complex]] | ||