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{{STRUCTURE_3ews|  PDB=3ews  |  SCENE=  }}
==Human DEAD-box RNA-helicase DDX19 in complex with ADP==
===Human DEAD-box RNA-helicase DDX19 in complex with ADP===
<StructureSection load='3ews' size='340' side='right' caption='[[3ews]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
{{ABSTRACT_PUBMED_19244245}}
== Structural highlights ==
<table><tr><td colspan='2'>[[3ews]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EWS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DBP5, DDX19, DDX19B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ews FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ews OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ews RCSB], [http://www.ebi.ac.uk/pdbsum/3ews PDBsum]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/3ews_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
DEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an alpha-helix that inserts between the conserved domains of the free protein to negatively regulate ATPase activity. This finding was corroborated by biochemical data that confirm an autoregulatory function of the N-terminal region of the protein. This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations.


==About this Structure==
The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch.,Collins R, Karlberg T, Lehtio L, Schutz P, van den Berg S, Dahlgren LG, Hammarstrom M, Weigelt J, Schuler H J Biol Chem. 2009 Apr 17;284(16):10296-300. Epub 2009 Feb 25. PMID:19244245<ref>PMID:19244245</ref>
[[3ews]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWS OCA].
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>


==See Also==
==See Also==
Line 10: Line 30:
*[[Dead-box RNA helicase DDX19%2C in complex with an ATP-analogue and RNA|Dead-box RNA helicase DDX19%2C in complex with an ATP-analogue and RNA]]
*[[Dead-box RNA helicase DDX19%2C in complex with an ATP-analogue and RNA|Dead-box RNA helicase DDX19%2C in complex with an ATP-analogue and RNA]]
*[[Helicase|Helicase]]
*[[Helicase|Helicase]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:019244245</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Andersson,j.]]
[[Category: Andersson,j]]
[[Category: Arrowsmith,c h.]]
[[Category: Arrowsmith,c h]]
[[Category: Berglund,h.]]
[[Category: Berglund,h]]
[[Category: Bountra, c.]]
[[Category: Bountra, c]]
[[Category: Collins,r.]]
[[Category: Collins,r]]
[[Category: Dahlgren,l g.]]
[[Category: Dahlgren,l g]]
[[Category: Edwards,a m.]]
[[Category: Edwards,a m]]
[[Category: Flodin,s.]]
[[Category: Flodin,s]]
[[Category: Flores,a.]]
[[Category: Flores,a]]
[[Category: Graslund,s.]]
[[Category: Graslund,s]]
[[Category: Hammarstrom,m.]]
[[Category: Hammarstrom,m]]
[[Category: Johansson,a.]]
[[Category: Johansson,a]]
[[Category: Johansson,i.]]
[[Category: Johansson,i]]
[[Category: Karlberg,t.]]
[[Category: Karlberg,t]]
[[Category: Kotenyova,t.]]
[[Category: Kotenyova,t]]
[[Category: Lehtio,l.]]
[[Category: Lehtio,l]]
[[Category: Moche,m.]]
[[Category: Moche,m]]
[[Category: Nilsson,m e.]]
[[Category: Nilsson,m e]]
[[Category: Nordlund,p.]]
[[Category: Nordlund,p]]
[[Category: Nyman,t.]]
[[Category: Nyman,t]]
[[Category: Olesen,k.]]
[[Category: Olesen,k]]
[[Category: Persson,c.]]
[[Category: Persson,c]]
[[Category: SGC, Structural Genomics Consortium.]]
[[Category: Structural genomic]]
[[Category: Sagemark,j.]]
[[Category: Sagemark,j]]
[[Category: Schueler,h.]]
[[Category: Schueler,h]]
[[Category: Thorsell,a g.]]
[[Category: Thorsell,a g]]
[[Category: Tresaugues,l.]]
[[Category: Tresaugues,l]]
[[Category: Weigelt, j.]]
[[Category: Weigelt, j]]
[[Category: Welin,m.]]
[[Category: Welin,m]]
[[Category: Wikstrom,m.]]
[[Category: Wikstrom,m]]
[[Category: Wisniewska,m.]]
[[Category: Wisniewska,m]]
[[Category: Berg, s Van den.]]
[[Category: Berg, s Van den]]
[[Category: Adp]]
[[Category: Adp]]
[[Category: Atp-binding]]
[[Category: Atp-binding]]
Line 62: Line 83:
[[Category: Rrna]]
[[Category: Rrna]]
[[Category: Sgc]]
[[Category: Sgc]]
[[Category: Structural genomic]]
[[Category: Structural genomics consortium]]
[[Category: Translocation]]
[[Category: Translocation]]
[[Category: Transport]]
[[Category: Transport]]

Revision as of 16:51, 17 December 2014

Human DEAD-box RNA-helicase DDX19 in complex with ADPHuman DEAD-box RNA-helicase DDX19 in complex with ADP

Structural highlights

3ews is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:DBP5, DDX19, DDX19B (Homo sapiens)
Resources:FirstGlance, OCA, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

DEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an alpha-helix that inserts between the conserved domains of the free protein to negatively regulate ATPase activity. This finding was corroborated by biochemical data that confirm an autoregulatory function of the N-terminal region of the protein. This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations.

The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch.,Collins R, Karlberg T, Lehtio L, Schutz P, van den Berg S, Dahlgren LG, Hammarstrom M, Weigelt J, Schuler H J Biol Chem. 2009 Apr 17;284(16):10296-300. Epub 2009 Feb 25. PMID:19244245[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Collins R, Karlberg T, Lehtio L, Schutz P, van den Berg S, Dahlgren LG, Hammarstrom M, Weigelt J, Schuler H. The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch. J Biol Chem. 2009 Apr 17;284(16):10296-300. Epub 2009 Feb 25. PMID:19244245 doi:10.1074/jbc.C900018200

3ews, resolution 2.70Å

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