Cas9: Difference between revisions
mNo edit summary |
mNo edit summary |
||
| Line 3: | Line 3: | ||
The CRISPR-associated endonuclease [[Cas9]] has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences. | The CRISPR-associated endonuclease [[Cas9]] has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences. | ||
[[Image:Layout for schematic and structure with structure.png]] | [[Image:Layout for schematic and structure with structure.png|600px]] | ||
Articles in Proteopedia concerning Cas9 include: | Articles in Proteopedia concerning Cas9 include: | ||