1opy: Difference between revisions

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Revision as of 14:14, 20 March 2008

File:1opy.jpg

, resolution 1.9Å

Sites:

Activity:

Steroid Delta-isomerase, with EC number 5.3.3.1

Coordinates:

save as pdb, mmCIF, xml

KSI

OverviewOverview

The three-dimensional structure of the enzyme 3-oxo-delta5-steroid isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by multidimensional heteronuclear magnetic resonance spectroscopy. The two independently folded monomers pack together by means of extensive hydrophobic and electrostatic interactions. Each monomer comprises three alpha helices and a six-strand mixed beta-pleated sheet arranged to form a deep hydrophobic cavity. Catalytically important residues Tyr14 (general acid) and Asp38 (general base) are located near the bottom of the cavity and positioned as expected from mechanistic hypotheses. An unexpected acid group (Asp99) is also located in the active site adjacent to Tyr14, and kinetic and binding studies of the Asp99 to Ala mutant demonstrate that Asp99 contributes to catalysis by stabilizing the intermediate.

About this StructureAbout this Structure

1OPY is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:9103200

Page seeded by OCA on Thu Mar 20 13:14:01 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1opy.jpg|left|200px]]<br /><applet load="1opy" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1opy.jpg|left|200px]]
-caption="1opy, resolution 1.9&Aring;" />
-'''KSI'''<br />
+ {{Structure
+|PDB= 1opy |SIZE=350|CAPTION= <scene name='initialview01'>1opy</scene>, resolution 1.9&Aring;
+|SITE= <scene name='pdbsite=A:Active+Site'>A</scene>
+|LIGAND=
+|ACTIVITY= [http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1]
+|GENE=
+}}
+'''KSI'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-OPY is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Active as [http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] Known structural/functional Site: <scene name='pdbsite=A:Active+Site'>A</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPY OCA].
+OPY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OPY OCA].
 ==Reference==
-Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9103200 9103200]
+Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9103200 9103200]
 [[Category: Pseudomonas putida]]
 [[Category: Single protein]]
@@ Line 28: / Line 37: @@
 [[Category: pseudomonas putida biotype b]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:20:18 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:14:01 2008''