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| ==Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1I, Contains 60S subunit. The 40S Ribosomal Subunit Is In File 1S1H.==
| | #REDIRECT [[4v4b]] This PDB entry is obsolete and replaced by 4v4b |
| <StructureSection load='1s1i' size='340' side='right' caption='[[1s1i]], [[Resolution|resolution]] 11.70Å' scene=''>
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| == Structural highlights ==
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| <table><tr><td colspan='2'>[[1s1i]] is a 29 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S1I FirstGlance]. <br>
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| </td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1k5x|1k5x]], [[1k5y|1k5y]], [[1mms|1mms]], [[1mzp|1mzp]], [[1ffk|1ffk]], [[1fjf|1fjf]], [[1fka|1fka]], [[1gix|1gix]], [[1giy|1giy]], [[1nou|1nou]], [[1s1h|1s1h]]</td></tr>
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| <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s1i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s1i RCSB], [http://www.ebi.ac.uk/pdbsum/1s1i PDBsum]</span></td></tr>
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| <table>
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| == Evolutionary Conservation ==
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| [[Image:Consurf_key_small.gif|200px|right]]
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| Check<jmol>
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| <jmolCheckbox>
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| <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s1/1s1i_consurf.spt"</scriptWhenChecked>
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| <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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| <text>to colour the structure by Evolutionary Conservation</text>
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| </jmolCheckbox>
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| </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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| <div style="clear:both"></div>
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| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction.
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| Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.,Spahn CM, Gomez-Lorenzo MG, Grassucci RA, Jorgensen R, Andersen GR, Beckmann R, Penczek PA, Ballesta JP, Frank J EMBO J. 2004 Mar 10;23(5):1008-19. Epub 2004 Feb 19. PMID:14976550<ref>PMID:14976550</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| ==See Also==
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| *[[Ribosome 3D structures|Ribosome 3D structures]]
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| == References ==
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| <references/>
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| __TOC__
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| </StructureSection>
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| [[Category: Saccharomyces cerevisiae]]
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| [[Category: Andersen, G R.]]
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| [[Category: Ballesta, J P.G.]]
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| [[Category: Beckmann, R.]]
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| [[Category: Frank, J.]]
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| [[Category: Gomez-Lorenzo, M G.]]
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| [[Category: Grassucci, R A.]]
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| [[Category: Jorgensen, R.]]
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| [[Category: Penczek, P A.]]
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| [[Category: Spahn, C M.]]
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| [[Category: 60s ribosomal subunit]]
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| [[Category: 80s ribosome]]
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| [[Category: Cryo-em]]
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| [[Category: Eef2]]
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| [[Category: Ribosome]]
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| [[Category: Sordarin]]
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| [[Category: Trna translocation]]
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