1oh4: Difference between revisions
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[[Image:1oh4.jpg|left|200px]] | [[Image:1oh4.jpg|left|200px]] | ||
'''STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE''' | {{Structure | ||
|PDB= 1oh4 |SIZE=350|CAPTION= <scene name='initialview01'>1oh4</scene>, resolution 1.35Å | |||
|SITE= <scene name='pdbsite=AC1:So4+Binding+Site+For+Chain+A'>AC1</scene> | |||
|LIGAND= <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1OH4 is a [ | 1OH4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OH4 OCA]. | ||
==Reference== | ==Reference== | ||
Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27., Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ, Structure. 2003 Jun;11(6):665-75. PMID:[http:// | Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27., Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ, Structure. 2003 Jun;11(6):665-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12791255 12791255] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Thermotoga maritima]] | [[Category: Thermotoga maritima]] | ||
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[[Category: mannan]] | [[Category: mannan]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:10:50 2008'' | ||
Revision as of 14:10, 20 March 2008
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| , resolution 1.35Å | |||||||
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| Ligands: | , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURAL AND THERMODYNAMIC DISSECTION OF SPECIFIC MANNAN RECOGNITION BY A CARBOHYDRATE-BINDING MODULE
OverviewOverview
The C-terminal 176 amino acids of a Thermotoga maritima mannanase (Man5) constitute a carbohydrate binding module (CBM) that has been classified into CBM family 27. The isolated CBM27 domain, named TmCBM27, binds tightly (K(a)s 10(5)-10(6) M(-1)) to beta-1, 4-mannooligosaccharides, carob galactomannan, and konjac glucomannan, but not to cellulose (insoluble and soluble) or soluble birchwood xylan. The X-ray crystal structures of native TmCBM27, a TmCBM27-mannohexaose complex, and a TmCBM27-6(3),6(4)-alpha-D-galactosyl-mannopentaose complex at 2.0 A, 1.6 A, and 1.35 A, respectively, reveal the basis of TmCBM27's specificity for mannans. In particular, the latter complex, which is the first structure of a CBM in complex with a branched plant cell wall polysaccharide, illustrates how the architecture of the binding site can influence the recognition of naturally substituted polysaccharides.
About this StructureAbout this Structure
1OH4 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
ReferenceReference
Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27., Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ, Structure. 2003 Jun;11(6):665-75. PMID:12791255
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