1wcv: Difference between revisions

Soj and Spo0J of the Gram-negative hyperthermophile Thermus thermophilus, belong to the conserved ParAB family of bacterial proteins implicated in, plasmid and chromosome partitioning. Spo0J binds to DNA near the, replication origin and localises at the poles following initiation of, replication. Soj oscillates in the nucleoid region in an ATP- and, Spo0J-dependent fashion. Here, we show that Soj undergoes ATP-dependent, dimerisation in solution and forms nucleoprotein filaments with DNA., Crystal structures of Soj in three nucleotide states demonstrate that the, empty and ADP-bound states are monomeric, while a hydrolysis-deficient, mutant, D44A, is capable of forming a nucleotide 'sandwich' dimer. Soj, ATPase activity is stimulated by Spo0J or the N-terminal 20 amino-acid, peptide of Spo0J. Our analysis shows that dimerisation and activation, involving a peptide containing a Lys/Arg is conserved for Soj, ParA and, MinD and their modulators Spo0J, ParB and MinE, respectively. By homology, to the nitrogenase iron protein and the GTPases Ffh/FtsY, we suggest that, Soj dimerisation and regulation represent a conserved biological switch.

1WCV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with CL and IOD as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: 1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WCV OCA].  

''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 12:28:06 2007''