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[[ | ==crystal structure of GFP-TYR151PYZ with an unnatural amino acid incorporation== | ||
<StructureSection load='4ges' size='340' side='right' caption='[[4ges]], [[Resolution|resolution]] 1.23Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ges]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GES FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=0WZ:3-(1H-PYRAZOL-1-YL)-L-TYROSINE'>0WZ</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gf6|4gf6]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 Aequorea victoria])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ges FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ges OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ges RCSB], [http://www.ebi.ac.uk/pdbsum/4ges PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
ET encounters jellyfish: Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu(II) ion was shown to occur within one nanosecond in a distance-dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to Cu(II) ions. | |||
Genetic incorporation of a metal-chelating amino Acid as a probe for protein electron transfer.,Liu X, Li J, Dong J, Hu C, Gong W, Wang J Angew Chem Int Ed Engl. 2012 Oct 8;51(41):10261-5. doi: 10.1002/anie.201204962., Epub 2012 Aug 31. PMID:22936654<ref>PMID:22936654</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[Green Fluorescent Protein|Green Fluorescent Protein]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Aequorea victoria]] | [[Category: Aequorea victoria]] | ||
[[Category: Dong, J | [[Category: Dong, J]] | ||
[[Category: Gong, W | [[Category: Gong, W]] | ||
[[Category: Li, J | [[Category: Li, J]] | ||
[[Category: Liu, X | [[Category: Liu, X]] | ||
[[Category: Wang, J | [[Category: Wang, J]] | ||
[[Category: Electron transduction]] | [[Category: Electron transduction]] | ||
[[Category: Fluorescence]] | [[Category: Fluorescence]] | ||
[[Category: Fluorescent protein]] | [[Category: Fluorescent protein]] | ||
Revision as of 12:57, 10 December 2014
crystal structure of GFP-TYR151PYZ with an unnatural amino acid incorporationcrystal structure of GFP-TYR151PYZ with an unnatural amino acid incorporation
Structural highlights
Publication Abstract from PubMedET encounters jellyfish: Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu(II) ion was shown to occur within one nanosecond in a distance-dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to Cu(II) ions. Genetic incorporation of a metal-chelating amino Acid as a probe for protein electron transfer.,Liu X, Li J, Dong J, Hu C, Gong W, Wang J Angew Chem Int Ed Engl. 2012 Oct 8;51(41):10261-5. doi: 10.1002/anie.201204962., Epub 2012 Aug 31. PMID:22936654[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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