4gzn: Difference between revisions

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-[[Image:4gzn.png|left|200px]]
+==Mouse ZFP57 zinc fingers in complex with methylated DNA==
+<StructureSection load='4gzn' size='340' side='right' caption='[[4gzn]], [[Resolution|resolution]] 0.99&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4gzn]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GZN FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Zfp57 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gzn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gzn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gzn RCSB], [http://www.ebi.ac.uk/pdbsum/4gzn PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Zinc finger transcription factor Zfp57 recognizes the methylated CpG within the TGCCGC element. We determined the structure of the DNA-binding domain of Zfp57, consisting of two adjacent zinc fingers, in complex with fully methylated DNA at 1.0 A resolution. The first zinc finger contacts the 5' half (TGC), and the second recognizes the 3' half (CGC) of the recognition sequence. Zfp57 recognizes the two 5-methylcytosines (5mCs) asymmetrically: One involves hydrophobic interactions with Arg178, which also interacts with the neighboring 3' guanine and forms a 5mC-Arg-G interaction, while the other involves a layer of ordered water molecules. Two point mutations in patients with transient neonatal diabetes abolish DNA-binding activity. Zfp57 has reduced binding affinity for unmodified DNA and the oxidative products of 5mC.
-{{STRUCTURE_4gzn|  PDB=4gzn  |  SCENE=  }}
+An atomic model of Zfp57 recognition of CpG methylation within a specific DNA sequence.,Liu Y, Toh H, Sasaki H, Zhang X, Cheng X Genes Dev. 2012 Nov 1;26(21):2374-9. doi: 10.1101/gad.202200.112. Epub 2012 Oct, 11. PMID:23059534<ref>PMID:23059534</ref>
-===Mouse ZFP57 zinc fingers in complex with methylated DNA===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_23059534}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[4gzn]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GZN OCA].
+</StructureSection>
 [[Category: Mus musculus]]
-[[Category: Cheng, X.]]
+[[Category: Cheng, X]]
-[[Category: Liu, Y.]]
+[[Category: Liu, Y]]
-[[Category: Zhang, X.]]
+[[Category: Zhang, X]]
 [[Category: Transcription-dna complex]]
 [[Category: Zinc finger]]