3uvu: Difference between revisions

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-[[Image:3uvu.png|left|200px]]
+==Structural basis of nuclear import of Flap endonuclease 1 (FEN1)==
+<StructureSection load='3uvu' size='340' side='right' caption='[[3uvu]], [[Resolution|resolution]] 2.38&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3uvu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3UVU FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pjn|1pjn]], [[3rzx|3rzx]], [[1pjm|1pjm]], [[3rz9|3rz9]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kpna2, Rch1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3uvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3uvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3uvu RCSB], [http://www.ebi.ac.uk/pdbsum/3uvu PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Flap endonuclease 1 (FEN1) is a member of the nuclease family and is structurally conserved from bacteriophages to humans. This protein is involved in multiple DNA-processing pathways, including Okazaki fragment maturation, stalled replication-fork rescue, telomere maintenance, long-patch base-excision repair and apoptotic DNA fragmentation. FEN1 has three functional motifs that are responsible for its nuclease, PCNA-interaction and nuclear localization activities, respectively. It has been shown that the C-terminal nuclear localization sequence (NLS) facilitates nuclear localization of the enzyme during the S phase of the cell cycle and in response to DNA damage. To determine the structural basis of the recognition of FEN1 by the nuclear import receptor importin alpha, the crystal structure of the complex of importin alpha with a peptide corresponding to the FEN1 NLS was solved. Structural studies confirmed the binding of the FEN1 NLS as a classical bipartite NLS; however, in contrast to the previously proposed (354)KRKX(8)KKK(367) sequence, it is the (354)KRX(10)KKAK(369) sequence that binds to importin alpha. This result explains the incomplete inhibition of localization that was observed on mutating residues (365)KKK(367). Acidic and polar residues in the X(10) linker region close to the basic clusters play an important role in binding to importin alpha. These results suggest that the basic residues in the N-terminal basic cluster of bipartite NLSs may play roles that are more critical than those of the many basic residues in the C-terminal basic cluster.
-{{STRUCTURE_3uvu|  PDB=3uvu  |  SCENE=  }}
+Structural basis of nuclear import of flap endonuclease 1 (FEN1).,de Barros AC, Takeda AA, Chang CW, Kobe B, Fontes MR Acta Crystallogr D Biol Crystallogr. 2012 Jul;68(Pt 7):743-50. doi:, 10.1107/S0907444912010281. Epub 2012 Jun 15. PMID:22751659<ref>PMID:22751659</ref>
-===Structural basis of nuclear import of Flap endonuclease 1 (FEN1)===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_22751659}}
+==See Also==
+*[[Importin|Importin]]
-==About this Structure==
+== References ==
-[[3uvu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3UVU OCA].
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Mus musculus]]
-[[Category: Barros, A C.]]
+[[Category: Barros, A C]]
-[[Category: Fontes, M R.M.]]
+[[Category: Fontes, M R.M]]
-[[Category: Takeda, A A.S.]]
+[[Category: Takeda, A A.S]]
 [[Category: Fen 1]]
 [[Category: Flap endonuclease 1]]
 [[Category: Protein binding-peptide complex]]