3vrg: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[ | ==The crystal structure of hemoglobin from woolly mammoth in the met form== | ||
<StructureSection load='3vrg' size='340' side='right' caption='[[3vrg]], [[Resolution|resolution]] 1.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3vrg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mammuthus_primigenius Mammuthus primigenius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VRG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VRG FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vfe|3vfe]], [[3vff|3vff]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HBA-T2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37349 Mammuthus primigenius]), HBB/D ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37349 Mammuthus primigenius])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vrg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vrg RCSB], [http://www.ebi.ac.uk/pdbsum/3vrg PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The haemoglobin (Hb) of the extinct woolly mammoth has been recreated using recombinant genes expressed in Escherichia coli. The globin gene sequences were previously determined using DNA recovered from frozen cadavers. Although highly similar to the Hb of existing elephants, the woolly mammoth protein shows rather different responses to chloride ions and temperature. In particular, the heat of oxygenation is found to be much lower in mammoth Hb, which appears to be an adaptation to the harsh high-latitude climates of the Pleistocene Ice Ages and has been linked to heightened sensitivity of the mammoth protein to protons, chloride ions and organic phosphates relative to that of Asian elephants. To elucidate the structural basis for the altered homotropic and heterotropic effects, the crystal structures of mammoth Hb have been determined in the deoxy, carbonmonoxy and aquo-met forms. These models, which are the first structures of Hb from an extinct species, show many features reminiscent of human Hb, but underline how the delicate control of oxygen affinity relies on much more than simple overall quaternary-structure changes. | |||
Structures of haemoglobin from woolly mammoth in liganded and unliganded states.,Noguchi H, Campbell KL, Ho C, Unzai S, Park SY, Tame JR Acta Crystallogr D Biol Crystallogr. 2012 Nov;68(Pt 11):1441-9. doi:, 10.1107/S0907444912029459. Epub 2012 Oct 18. PMID:23090393<ref>PMID:23090393</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
== | *[[Hemoglobin 3D structures|Hemoglobin 3D structures]] | ||
[[ | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Mammuthus primigenius]] | [[Category: Mammuthus primigenius]] | ||
[[Category: Campbell, K L | [[Category: Campbell, K L]] | ||
[[Category: Ho, C | [[Category: Ho, C]] | ||
[[Category: Noguchi, H | [[Category: Noguchi, H]] | ||
[[Category: Park, S Y | [[Category: Park, S Y]] | ||
[[Category: Tame, J R.H | [[Category: Tame, J R.H]] | ||
[[Category: Oxygen storage/transport]] | [[Category: Oxygen storage/transport]] | ||
[[Category: Oxygen transport]] | [[Category: Oxygen transport]] | ||
[[Category: Woolly mammoth hemoglobin]] | [[Category: Woolly mammoth hemoglobin]] | ||